The leucine metabolite 3-hydroxy-3-methylbutyrate (HMB) modifies protein turnover in muscles of laboratory rats and domestic chickens in vitro
Autor: | P. Ostaszewski, B. Balasinska, F. Glomot, I. Papet, S. Kostiuk, Michał Jank |
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Přispěvatelé: | Warsaw Agricultural University, Unité de Nutrition Humaine (UNH), Institut National de la Recherche Agronomique (INRA)-Université d'Auvergne - Clermont-Ferrand I (UdA)-Clermont Université |
Jazyk: | angličtina |
Rok vydání: | 2000 |
Předmět: |
0303 health sciences
medicine.medical_specialty 030309 nutrition & dietetics [SDV]Life Sciences [q-bio] 0402 animal and dairy science Protein turnover Phenylalanine 04 agricultural and veterinary sciences Metabolism Biology Protein degradation musculoskeletal system 040201 dairy & animal science 03 medical and health sciences Protein catabolism Endocrinology Food Animals Internal medicine medicine Protein biosynthesis Animal Science and Zoology Tyrosine Leucine |
Zdroj: | Journal of Animal Physiology and Animal Nutrition Journal of Animal Physiology and Animal Nutrition, Wiley, 2000, 84 (1-2), pp.1-8. ⟨10.1046/j.1439-0396.2000.00272.x⟩ |
ISSN: | 0931-2439 1439-0396 |
Popis: | International audience; This study was conducted to assess the in vitro effects of leucine and 3‐hydroxy‐3‐methylbutyrate (HMB) on protein synthesis and degradation in isolated rat and chick muscles. Isolated muscles — extensor digitorum longus (EDL) and soleus (S) in rats and EDL and tibialis anterior (TA) in chicks — were taken out intact and incubated in Krebs—Henseleit buffer with [14C]phenylalanine. Protein synthesis was then determined by measuring [14C]phenylalanine incorporation into protein and protein breakdown was estimated by net release of tyrosine from muscle proteins. In all muscles investigated leucine stimulated protein synthesis (average 20%; p < 0.05 versus control) but only slightly reduced proteolysis (average 8%, NS). In contrast, HMB had no significant effect on protein synthesis but consistently decreased protein degradation in the range from −29% (p < 0.01 versus control) in rat extensor digitorus longus to −5% (NS) in rat soleus muscles. HMB appeared to suppress protein degradation more in white muscle fibres than in red muscle fibres (at least in the rat). These data support observations in humans indicating that HMB decreases muscle proteolysis. It is suggested that metabolism of leucine to HMB could be responsible for the reported suppressive effects of leucine on protein breakdown. |
Databáze: | OpenAIRE |
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