Flow Cytometry, HPLC-RP, and metAFLP Analyses to Assess Genetic Variability in Somatic Embryo-Derived Plantlets of Gentiana pannonica Scop

Autor:	Agnieszka Fiuk, Piotr T. Bednarek, Jan J. Rybczyński
Rok vydání:	2010
Předmět:	Somatic embryogenesis Plant Science Methylation Biology biology.organism_classification Molecular biology chemistry.chemical_compound chemistry Molecular marker DNA methylation sense organs Genetic variability Epigenetics Ploidy Molecular Biology Gentiana
Zdroj:	National Information Processing Institute
ISSN:	1572-9818 0735-9640
DOI:	10.1007/s11105-009-0167-3
Popis:	Cytometric and molecular techniques were used to verify genetic uniformity among somatic embryo-derived plantlets of Gentiana pannonica Scop. Cytometric analysis of regenerants revealed absence of chromosomal changes and alterations in ploidy. However, reverse phase high pressure liquid chromatography detected higher levels of methylation in regenerated plants than those of control plants. These changes were further investigated using a quantitative molecular marker-based approach. This revealed that numerous tissue culture-induced variations, ∼3% (epi)mutations, were observed, including sequence variation and changes in methylation patterns. Moreover, complex patterns of variation, including combinations of genetic and epigenetic changes, were relatively high (ca. 9%). Overall, tissue culture-induced variation reached 16%; while, demethylation was lower than de novo methylation in heterozygotic material and similar in all regenerated plantlets.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d57ce985a6934c802fe21682bfc07379 https://doi.org/10.1007/s11105-009-0167-3 Zobrazit plný text záznamu Plný text ve formátu PDF