Modulation of Nitrosative Stress via Glutathione-Dependent Formaldehyde Dehydrogenase and S-Nitrosoglutathione Reductase
| Autor: | Jenq-Kuen Huang, Chuian-Fu Ken, Chi-Tsai Lin, Lisa Wen, Chih-Yu Huang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
S-nitrosoglutathione (GSNO)
Apoptosis Reductase Biology Catalysis Article Cell Line Inorganic Chemistry lcsh:Chemistry chemistry.chemical_compound Humans Physical and Theoretical Chemistry Molecular Biology lcsh:QH301-705.5 Spectroscopy Formaldehyde dehydrogenase chemistry.chemical_classification Organic Chemistry HEK 293 cells S-nitrosoglutathione reductase (GSNOR) glutathione-dependent formaldehyde dehydrogenase (GFD) General Medicine Transfection Glutathione Taiwanofungus camphorata (formerly named Antrodia camphorata) Molecular biology Aldehyde Oxidoreductases Computer Science Applications Enzyme Activation Enzyme chemistry lcsh:Biology (General) lcsh:QD1-999 Cell culture S-Nitrosoglutathione |
| Zdroj: | International Journal of Molecular Sciences Volume 15 Issue 8 Pages 14166-14179 International Journal of Molecular Sciences, Vol 15, Iss 8, Pp 14166-14179 (2014) |
| ISSN: | 1422-0067 |
| Popis: | Glutathione-dependent formaldehyde dehydrogenase (GFD) from Taiwanofungus camphorata plays important roles in formaldehyde detoxification and antioxidation. The enzyme is bifunctional. In addition to the GFD activity, it also functions as an effective S-nitrosoglutathione reductase (GSNOR) against nitrosative stress. We investigated the modulation of HEK (human embryonic kidney) 293T cells under nitrosative stress by transfecting a codon optimized GFD cDNA from Taiwanofungus camphorata (Tc-GFD-O) to these cells. The parental and transfected HEK 293T cells were then subjected to S-nitrosoglutathione treatment to induce nitrosative stress. The results showed that in Tc-GFD-O-transfected 293T cells, the expression and activity of GFD increased. Additionally, these cells under the nitrosative stress induced by S-nitrosoglutathione showed both higher viability and less apoptosis than the parental 293T cells. This finding suggests that the Tc-GFD-O in HEK 293T cells may provide a protective function under nitrosative stress. |
| Databáze: | OpenAIRE |
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