Actin network architecture can ensure robust centering or sensitive decentering of the centrosome

Autor: Shohei Yamamoto, Jérémie Gaillard, Benoit Vianay, Christophe Guerin, Magali Orhant‐Prioux, Laurent Blanchoin, Manuel Théry
Přispěvatelé: CytoMorphoLab, Physiologie cellulaire et végétale (LPCV), Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Grenoble Alpes (UGA)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Grenoble Alpes (UGA), Immunologie humaine, physiopathologie & immunothérapie (HIPI (UMR_S_976 / U976)), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité), Fellowships from the EMBO (ALTF 652-2019), Astellas Foundation for research on metabolic disorders, Mochida memorial foundation for medical and pharmaceutical research, ANR-17-EURE-0003,CBH-EUR-GS,CBH-EUR-GS(2017), European Project: 771599,ICEBERG, European Project: 741773,AAA, Martin-Laffon, Jacqueline, CBH-EUR-GS - - CBH-EUR-GS2017 - ANR-17-EURE-0003 - EURE - VALID, Exploration below the tip of the microtubule - ICEBERG - 771599 - INCOMING, Adaptive Actin Architectures - AAA - 741773 - INCOMING
Rok vydání: 2022
Předmět:
Zdroj: EMBO Journal
EMBO Journal, 2022, 41 (20), pp.e111631. ⟨10.15252/embj.2022111631⟩
ISSN: 1460-2075
0261-4189
DOI: 10.15252/embj.2022111631⟩
Popis: International audience; The orientation of cell polarity depends on the position of the centrosome, the main microtubule-organizing center (MTOC). Microtubules (MTs) transmit pushing forces to the MTOC as they grow against the cell periphery. How the actin network regulates these forces remains unclear. Here, in a cell-free assay, we used purified proteins to reconstitute the interaction of a microtubule aster with actin networks of various architectures in cell-sized microwells. In the absence of actin filaments, MTOC positioning was highly sensitive to variations in microtubule length. The presence of a bulk actin network limited microtubule displacement, and MTOCs were held in place. In contrast, the assembly of a branched actin network along the well edges centered the MTOCs by maintaining an isotropic balance of pushing forces. An anisotropic peripheral actin network caused the MTOC to decenter by focusing the pushing forces. Overall, our results show that actin networks can limit the sensitivity of MTOC positioning to microtubule length and enforce robust MTOC centering or decentering depending on the isotropy of its architecture.
Databáze: OpenAIRE
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