Grape seed proanthocyanidins inhibit the proliferation, migration and invasion of tongue squamous cell carcinoma cells through suppressing the protein kinase?B/nuclear factor-κB signaling pathway
| Autor: | Jing Gao, Cuilan Hou, Yuan Zhang, Mengrou Xu, Yaya Yang, Shuangsheng Huang, Yanxin Qiu, Ninggang Yang, Xin Cheng |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
proliferation Cell nuclear factor-κB IκB kinase tongue squamous cell carcinoma Biology migration 03 medical and health sciences 0302 clinical medicine Cell Movement Cell Line Tumor Genetics medicine Humans Neoplasm Invasiveness Proanthocyanidins Protein kinase B Cell Proliferation Grape Seed Extract Oncogene apoptosis NF-kappa B Articles General Medicine Cell cycle invasion Tongue Neoplasms Cell biology Gene Expression Regulation Neoplastic 030104 developmental biology medicine.anatomical_structure Apoptosis Cell culture 030220 oncology & carcinogenesis grape seed proanthocyanidins Carcinoma Squamous Cell protein kinase B Signal transduction Proto-Oncogene Proteins c-akt Signal Transduction |
| Zdroj: | International Journal of Molecular Medicine |
| ISSN: | 1791-244X 1107-3756 |
| DOI: | 10.3892/ijmm.2017.3162 |
| Popis: | Tongue squamous cell carcinoma (TSCC) is the most common oral squamous cell carcinoma. Despite significant advances in combined therapies, the 5-year survival rate of patients with TSCC has not notably improved; this is due to regional recurrences and lymph node metastasis. Grape seed proanthocyanidins (GSPs) are consumed as dietary supplements worldwide and possess anticancer activity against several different types of cancer. However, their effect on TSCC and the underlying mechanisms by which they function remain unclear. In the present study, it was identified that GSPs significantly inhibited the viability and induced the apoptosis of Tca8113 cells in a dose-dependent manner. This was associated with a significantly increased expression of the pro-apoptosis regulator BAX protein and a significantly decreased expression of the anti-apoptosis regulator Bcl-2 protein at 100 µg/ml GSPs. In addition, at non-toxic concentrations GSPs significantly inhibited the secretion of matrix metalloproteinase-2 (MMP-2) and MMP-9 from Tca8113 cells, as well as their migration and invasion. Furthermore, it was demonstrated that GSPs significantly inhibited the phosphorylation of protein kinase B (Akt) and IκB kinase, as well as the translocation of nuclear factor-κB (NF-κB) into the nucleus of Tca8113 cells. Taken together, these results suggest that GSPs inhibit the proliferation, migration and invasion of Tca8113 cells through suppression of the Akt/NF-κB signaling pathway. This indicates that GSPs may be developed as a novel potential chemopreventive agent against TSCC. |
| Databáze: | OpenAIRE |
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