Immunohistochemical localization of tryptophan hydroxylase and serotonin transporter in the carotid body of the rat
| Autor: | Yoshio Yamamoto, Yamaguchi-Yamada Misuzu, Takuya Yokoyama |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Male
medicine.medical_specialty Histology Tryptophan Hydroxylase Biology Glomus cell Internal medicine medicine Animals RNA Messenger Rats Wistar Molecular Biology Serotonin transporter Serotonin Plasma Membrane Transport Proteins Carotid Body TPH1 TPH2 Reverse Transcriptase Polymerase Chain Reaction Cell Biology Tryptophan hydroxylase Immunohistochemistry Rats Isoenzymes Medical Laboratory Technology Endocrinology medicine.anatomical_structure biology.protein Carotid body Serotonin Acetylcholine medicine.drug |
| Zdroj: | Histochemistry and Cell Biology. 140:147-155 |
| ISSN: | 1432-119X 0948-6143 |
| DOI: | 10.1007/s00418-012-1066-5 |
| Popis: | It has been proposed that serotonin (5-HT) facilitates the chemosensory activity of the carotid body (CB). In the present study, we investigated mRNA expression and immunohistochemical localization of the 5-HT synthetic enzyme isoforms, tryptophan hydroxylase 1 (TPH1) and TPH2, and the 5-HT plasma membrane transport protein, 5-HT transporter (SERT), in the CB of the rat. RT-PCR analysis detected the expression of mRNA for TPH1 and SERT in extracts of the CB. Using immunohistochemistry, 5-HT immunoreactivity was observed in a few glomus cells. TPH1 and SERT immunoreactivities were observed in almost all glomus cells. SERT immunoreactivity was seen on nerve fibers with TPH1 immunoreactivity. SERT immunoreactivity was also observed in varicose nerve fibers immunoreactive for dopamine beta-hydroxylase, but not in nerve fibers immunoreactive for vesicular acetylcholine transporters or nerve terminals immunoreactive for P2X3 purinoreceptors. These results suggest that 5-HT is synthesized and released from glomus cells and sympathetic nerve fibers in the CB of the rat, and that the chemosensory activity of the CB is regulated by 5-HT from glomus cells and sympathetic nerve fibers. |
| Databáze: | OpenAIRE |
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