Development of a high-content imaging assay for screening compound aggregation
| Autor: | William G. Bonnette, Jeffrey W. Gross, Geoffrey Quinque, Ann F. Hoffman, Lawrence M. Szewczuk, Yong Jiang, Marc A. Holbert |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Surface Properties Cell Biophysics Protein aggregation 010402 general chemistry 01 natural sciences Biochemistry 03 medical and health sciences Protein Aggregates Optical imaging High-Throughput Screening Assays medicine Humans Particle Size Molecular Biology Fluorescent Dyes Chemistry Optical Imaging Proteins Cell Biology Small molecule 0104 chemical sciences 030104 developmental biology medicine.anatomical_structure Protein target |
| Zdroj: | Analytical biochemistry. 559 |
| ISSN: | 1096-0309 |
| Popis: | Aggregated compounds can promiscuously and nonspecifically associate with proteins resulting in either false inhibition or activation of many different protein target classes. We developed a high-content imaging assay in a 384-well format using fluorescently labeled target proteins and an Operetta cell imager to screen for compound aggregates that interact with target proteins. The high-throughput assay can not only directly detect the interaction between compound aggregators and the target of interest, but also determine the critical aggregation concentration (CAC) of a given promiscuous small molecule. |
| Databáze: | OpenAIRE |
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