Phenotypic and genotypic characteristics of Arcanobacterium haemolyticum isolated from clinical samples in a Danish hospital
| Autor: | Ellen Prenger-Berninghoff, Osama Sammra, Samy Nagib, M. Hijazin, Amir Abdulmawjood, Christoph Lämmler, Alice Friis-Møller, Markus Timke, J. Alber, Anna Balbutskaya, Markus Kostrzewa |
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| Rok vydání: | 2014 |
| Předmět: |
DNA
Bacterial Virulence Factors Denmark Molecular Sequence Data Virulence Arcanobacterium haemolyticum DNA Ribosomal Microbiology CAMP test RNA Ribosomal 16S Genotype Cluster Analysis Humans Gene Phylogeny Genetics biology Hemolysin Sequence Analysis DNA General Medicine 16S ribosomal RNA biology.organism_classification Arcanobacterium Hospitals Bacterial Typing Techniques RNA Ribosomal 23S Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization biology.protein DNA Intergenic Actinomycetales Infections Neuraminidase |
| Zdroj: | Folia Microbiologica. 59:369-374 |
| ISSN: | 1874-9356 0015-5632 |
| DOI: | 10.1007/s12223-014-0308-4 |
| Popis: | Six Arcanobacterium haemolyticum strains isolated from six patients of two hospitals in Denmark were identified phenotypically, also including matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis, and by genotypic methods. The latter were performed by sequencing 16S rDNA and glyceraldehyde 3-phosphate dehydrogenase encoding gene gap and by amplification of an A. haemolyticum specific region of 16S-23S rDNA intergenic spacer region and 23S rDNA. The six A. haemolyticum strains were further investigated for the presence of seven potential virulence genes encoding arcanolysin, phospholipase D, hemolysin A, CAMP factor family protein, collagen binding protein, neuraminidase A and neuraminidase H which appeared to be present in two (seven virulence genes), two (six virulence genes) and two strains (four virulence genes), respectively. The phenotypic and genotypic properties described in the present study might help to reliably identify and further characterize A. haemolyticum isolated from human patients, a species which seems to be of increasing importance. |
| Databáze: | OpenAIRE |
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