Mechanism of a Disassembly-Driven Sensing System Studied by Stopped-Flow Kinetics

Autor:	Cara Gallo, Suma S. Thomas, Fraser Hof, Allison J Selinger, Cornelia Bohne
Rok vydání:	2021
Předmět:	010405 organic chemistry Sensing applications Chemistry Organic Chemistry Kinetics 010402 general chemistry Stopped flow 01 natural sciences Fluorescence 0104 chemical sciences Mechanism (engineering) Self-assembly Biological system Sensing system
Zdroj:	The Journal of Organic Chemistry. 86:10782-10787
ISSN:	1520-6904 0022-3263
DOI:	10.1021/acs.joc.1c00959
Popis:	We carried out steady-state and stopped-flow photophysical measurements to determine the kinetics of a discrete disassembly driven turn-on fluorescent system. On and off rates for both DimerDye1 assembly and nicotine binding were determined. Relative rates for these competing processes provide insight on how this system can be optimized for sensing applications. Kinetics studies in artificial saliva showed that moving to more complex media has minimal effects on the sensing ability of the system.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d5723aa6164b93947e4e0f654a10a323 https://doi.org/10.1021/acs.joc.1c00959 Zobrazit plný text záznamu