Accurate and simple determination of oxcarbazepine in human plasma and urine samples using switchable‐hydrophilicity solvent in GC–MS
| Autor: | Fatma Turak, Omer Tahir Gunkara, Sezin Erarpat, Sezgin Bakırdere, Süleyman Bodur, Dotse Selali Chormey, Merve Fırat Ayyıldız, Fatih Ahmet Erulaş, Türkan Börklü Budak |
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| Rok vydání: | 2020 |
| Předmět: |
Analyte
Base (chemistry) Calibration curve Clinical Biochemistry Oxcarbazepine 030226 pharmacology & pharmacy 01 natural sciences Biochemistry Gas Chromatography-Mass Spectrometry Analytical Chemistry 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Limit of Detection Drug Discovery Humans Molecular Biology Pharmacology Detection limit chemistry.chemical_classification Chromatography Chemistry 010401 analytical chemistry Reproducibility of Results General Medicine 0104 chemical sciences Solvent Volume (thermodynamics) Sodium hydroxide Linear Models Solvents Gas chromatography–mass spectrometry Hydrophobic and Hydrophilic Interactions |
| Zdroj: | Biomedical Chromatography. 34 |
| ISSN: | 1099-0801 0269-3879 |
| DOI: | 10.1002/bmc.4915 |
| Popis: | This work presents a sensitive and rapid analytical method for the determination of oxcarbazepine in human plasma and urine samples. A vortex-assisted switchable hydrophilicity solvent-based liquid phase microextraction (VA-SHS-LPME) was used to preconcentrate oxcarbazepine from the samples before the determination by gas chromatography mass spectrometry. The switchable hydrophilicity solvent was synthesized by protonating N,N-dimethylbenzylamine with carbon dioxide to make it totally miscible with an equivalent volume of water. Parameters of the VA-SHS-LPME method including volume of switchable hydrophilicity solvent, concentration/volume of sodium hydroxide and vortex period were systematically optimized. Under the optimum conditions, good linearity ranging from 27.03 to 353.47 μg/kg was obtained for the analyte. Limit of detection and quantitation values were found to be 6.2 and 21 μg/kg (mass base), respectively. The relative standard deviation was calculated as 6.9% for six replicate measurements of the lowest concentration of the calibration plot. Satisfactory recovery results were calculated in the range of 97-100% for human plasma and urine samples spiked at five different concentrations. |
| Databáze: | OpenAIRE |
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