The role of Carboxydothermus hydrogenoformans in the conversion of calcium phosphate from amorphous to crystalline state
| Autor: | Hojatollah Vali, Jeanne Paquette, Mathieu Haddad, Serge R. Guiot |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Calcium Phosphates
Biomineralization Anatomy and Physiology Applied Microbiology Nucleation lcsh:Medicine Thermoanaerobacter Carboxydothermus hydrogenoformans magnesium Crystallography X-Ray biofilm calcium phosphate law.invention nanocrystal bacteriolysis law Microbial Physiology Spectroscopy Fourier Transform Infrared Amorphous calcium phosphate Crystallization infrared spectroscopy lcsh:Science Dissolution Multidisciplinary biology Chemistry hydroxyapatite Agriculture Biogeochemistry nanorod Whitlockite Medicine Research Article Biotechnology X ray diffraction Materials Science Material Properties Mineralogy bicarbonate Crystal growth engineering.material Microbiology Biomaterials Industrial Microbiology Microscopy Electron Transmission complex formation hyperthermophilic bacterium Mechanical Properties Biology electron microscopy lcsh:R Spectrometry X-Ray Emission biology.organism_classification Energy and Power Geochemistry Chemical engineering Biofuels Microscopy Electron Scanning Earth Sciences engineering lcsh:Q Physiological Processes |
| Zdroj: | PLoS ONE, Vol 9, Iss 2, p e89480 (2014) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | Two previously unknown modes of biomineralization observed in the presence of Carboxydothermus hydrogenoformans are presented. Following the addition of NaHCO3 and the formation of an amorphous calcium phosphate precipitate in a DSMZ medium inoculated with C. hydrogenoformans , two distinct crystalline solids were recovered after 15 and 30 days of incubation. The first of these solids occurred as micrometric clusters of blocky, angular crystals, which were associated with bacterial biofilm. The second solid occurred as 30-50 nm nanorods that were found scattered among the organic products of bacterial lysis. The biphasic mixture of solids was clearly dominated by the first phase. The X-ray diffractometry (XRD) peaks and Fourier transform infrared spectroscopy (FTIR) spectrum of this biphasic material consistently showed features characteristic of Mg-whitlockite. No organic content or protein could be identified by dissolving the solids. In both cases, the mode of biomineralization appears to be biologically induced rather than biologically controlled. Since Mg is known to be a strong inhibitor of the nucleation and growth of CaP, C. hydrogenoformans may act by providing sites that chelate Mg or form complexes with it, thus decreasing its activity as nucleation and crystal growth inhibitor. The synthesis of whitlockite and nano-HAP-like material by C. hydrogenoformans demonstrates the versatility of this organism also known for its ability to perform the water-gas shift reaction, and may have applications in bacterially mediated synthesis of CaP materials, as an environmentally friendly alternative process. © 2014 Haddad et al. |
| Databáze: | OpenAIRE |
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