Impact of CRFR1 Ablation on Amyloid-β Production and Accumulation in a Mouse Model of Alzheimer's Disease
| Autor: | Nickey Lee, Louise Monte, Michael C. Donohue, Kathleen U. Lao, Allyson D. Roe, Shannon N. Campbell, Cheng Zhang, Robert A. Rissman |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Genetically modified mouse
medicine.medical_specialty Transgene Hippocampus Mice Transgenic Plaque Amyloid Neuropathology Biology Receptors Corticotropin-Releasing Hormone Article Presenilin Transgenic Model Amyloid beta-Protein Precursor Alzheimer Disease Internal medicine Presenilin-1 medicine Animals Humans Receptor Mice Knockout Amyloid beta-Peptides General Neuroscience Brain General Medicine medicine.disease Mice Inbred C57BL Disease Models Animal Psychiatry and Mental health Clinical Psychology Endocrinology Female Geriatrics and Gerontology Alzheimer's disease |
| Zdroj: | Journal of Alzheimer's Disease. 45:1175-1184 |
| ISSN: | 1875-8908 1387-2877 |
| DOI: | 10.3233/jad-142844 |
| Popis: | Stress exposure and the corticotropin-releasing factor (CRF) system have been implicated as mechanistically involved in both Alzheimer's disease (AD) and associated rodent models. In particular, the major stress receptor, CRF receptor type 1 (CRFR1), modulates cellular activity in many AD-relevant brain areas, and has been demonstrated to impact both tau phosphorylation and amyloid-β (Aβ) pathways. The overarching goal of our laboratory is to develop and characterize agents that impact the CRF signaling system as disease-modifying treatments for AD. In the present study, we developed a novel transgenic mouse to determine whether partial or complete ablation of CRFR1 was feasible in an AD transgenic model and whether this type of treatment could impact Aβ pathology. Double transgenic AD mice (PSAPP) were crossed to mice null for CRFR1; resultant CRFR1 heterozygous (PSAPP-R1(+/-)) and homozygous (PSAPP-R1(-/-)) female offspring were used at 12 months of age to examine the impact of CRFR1 disruption on the severity of AD Aβ levels and pathology. We found that both PSAPP-R1(+/-) and PSAPP-R1(-/-) had significantly reduced Aβ burden in the hippocampus, insular, rhinal, and retrosplenial cortices. Accordingly, we observed dramatic reductions in Aβ peptides and AβPP-CTFs, providing support for a direct relationship between CRFR1 and Aβ production pathways. In summary, our results suggest that interference of CRFR1 in an AD model is tolerable and is efficacious in impacting Aβ neuropathology. |
| Databáze: | OpenAIRE |
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