Salacia mulbarica leaf extract mediated synthesis of silver nanoparticles for antibacterial and ct‐DNA damage via releasing of reactive oxygen species
| Autor: | K. S. V. Krishna Rao, Chandra Sekhar Espenti, Akila Chandra Sekhar, Kummara Madhusudana Rao, P. Ramesh |
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| Rok vydání: | 2020 |
| Předmět: |
Silver
DNA damage Metal Nanoparticles Nanoparticle Microbial Sensitivity Tests 02 engineering and technology 010402 general chemistry 01 natural sciences Silver nanoparticle Salacia Dynamic light scattering Electrical and Electronic Engineering Surface plasmon resonance Bacteria biology Plant Extracts Chemistry DNA 021001 nanoscience & nanotechnology biology.organism_classification Anti-Bacterial Agents 0104 chemical sciences Electronic Optical and Magnetic Materials Plant Leaves DNA fragmentation Reactive Oxygen Species 0210 nano-technology Antibacterial activity DNA Damage Research Article Biotechnology Nuclear chemistry |
| Zdroj: | IET Nanobiotechnol |
| ISSN: | 1751-875X 1751-8741 |
| Popis: | In this examination, we researched the advantages of DNA fragmentation and metallic nanoparticles well‐appointed with biomolecules. A novel interpretation of DNA damage by Silver Nano‐Clusters (AgNCs) which were developed by the utilization of green synthesis method was demonstrated. The green synthesis of AgNCs was accomplished by utilizing the leaf extract of Salacia mulbarica (SM). The preparation of SM‐AgNCs was developed by estimating surface plasmon resonance peak around 449 nm by using a UV–Visible spectrophotometer. The effect of phytochemicals in SM leaf extract on the development of stable SM‐AgNCs was confirmed by FTIR spectroscopy. The size of the fabricated SM‐AgNCs was estimated by dynamic light scattering and zeta‐sizer analysis and the morphology of the SM‐AgNCs was examined by transmission electron microscopy. The presence of clusters of Ag particles in the prepared SM‐AgNCs was recognized by energy dispersion X‐ray analysis. The results show that saponins, phytosterols, and phenolic compounds present in plant extract may play a great part in developing the SM‐AgNCs in their specialized particles. The succeeded SM‐AgNCs shows incredible anti‐bacterial action towards Escherichia coli and Bacillus subtilis. In‐light of the antibacterial study, these SM‐AgNCs were analyzed with calf thymus‐DNA and found significant damage to the strand of thymus‐DNA. |
| Databáze: | OpenAIRE |
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