Role of the major determinant of polar flagellation FlhG in the endoflagella‐containing spirochete Leptospira

Autor: Gerald L. Murray, Célia Fontana, Jun Liu, Hugo Varet, Ben Adler, Ruben Halifa, Mathieu Picardeau, David R. Hendrixson, Shuaiqi Guo, Alejandro Buschiazzo, Jean Yves Coppée, Lenka Fule, Odile Sismeiro, Rachel Legendre
Přispěvatelé: Biologie des Spirochètes / Biology of Spirochetes, Institut Pasteur [Paris], Integrative Microbiology of Zoonotic Agents [Paris and Montevideo] (IMiZA), Institut Pasteur de Montevideo, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut Pasteur [Paris], Université de Paris (UP), Boehringer Ingelheim Pharma GmbH & Co. KG, Pôle Biomics (C2RT), Centre de Ressources et de Recherche Technologique - Center for Technological Resources and Research (C2RT), Institut Pasteur [Paris]-Institut Pasteur [Paris], Transcriptome et Epigénome (PF2), Hub Bioinformatique et Biostatistique - Bioinformatics and Biostatistics HUB, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Monash University [Clayton], University of Texas Southwestern Medical Center, Réseau International des Instituts Pasteur (RIIP), Yale University School of Medicine, This research was supported by ANR LEPTOMOVE (18-CE15-0027-01) and the Pasteur International Unit « Integrative Microbiology of Zoonotic Agents » (IMiZA) to MP, NIH R01AI065539 to DRH, and NIAID R01AI087946 to JL. This work is part of the PhD of Lenka Fule., We thank Olivier Gorgette (UBI platform, Institut Pasteur, Paris, France) and Ariel Mechaly (Plateforme de Cristallographie, Institut Pasteur, Paris, France) for help in electron microscopy and protein structure analysis, respectively. We thank Christophe Becavin for RNA-seq analysis, Sébastien Le Hu Nghia for genetic studies and Meng Shao and Qin Gong for their assistance in cryo-ET sample preparation and segmentation. We also thank David Šmajs for the gift of Borrelia DNA, ANR-18-CE15-0027,LEPTOMOVE,Mécanismes moléculaires de la motilité chez les spirochètes: le modèle de l'endoflagelle des leptospires(2018), Université Paris Cité (UPCité)-Microbiologie Intégrative et Moléculaire (UMR6047), Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut Pasteur [Paris] (IP), Université Paris Cité (UPCité), Institut Pasteur [Paris] (IP)-Institut Pasteur [Paris] (IP), Institut Pasteur [Paris] (IP), Institut Pasteur [Paris] (IP)-Université Paris Cité (UPCité), Yale School of Medicine [New Haven, Connecticut] (YSM)
Rok vydání: 2021
Předmět:
Zdroj: Molecular Microbiology
Molecular Microbiology, Wiley, 2021, ⟨10.1111/mmi.14831⟩
Molecular Microbiology, 2021, ⟨10.1111/mmi.14831⟩
ISSN: 1365-2958
0950-382X
Popis: International audience; Spirochetes can be distinguished from other bacteria by their spiral-shaped morphology and subpolar periplasmic flagella. This study focused on FlhF and FlhG, which control the spatial and numerical regulation of flagella in many exoflagellated bacteria, in the spirochete Leptospira. In contrast to flhF which seems to be essential in Leptospira, we demonstrated that flhG- mutants in both the saprophyte L. biflexa and the pathogen L. interrogans were less motile than the wild-type strains in gel-like environments but not hyperflagellated as reported previously in other bacteria. Cryo-electron tomography revealed that the distance between the flagellar basal body and the tip of the cell decreased significantly in the flhG- mutant in comparison to wild-type and complemented strains. Additionally, comparative transcriptome analyses of L. biflexa flhG- and wild-type strains showed that FlhG acts as a negative regulator of transcription of some flagellar genes. We found that the L. interrogans flhG- mutant was attenuated for virulence in the hamster model. Cross-species complementation also showed that flhG is not interchangeable between species. Our results indicate that FlhF and FlhG in Leptospira contribute to governing cell motility but our data support the hypothesis that FlhF and FlhG function differently in each bacterial species, including among spirochetes.
Databáze: OpenAIRE
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