C-reactive protein and natural IgM antibodies are activators of complement in a rat model of intestinal ischemia and reperfusion
| Autor: | Thomas M. van Gulik, Niubel Diaz Padilla, Hans W.M. Niessen, Ivo G. Schoots, M. Adrie W. Maas, Esther E. Posno Peltenburg, Annebeth de Vries, C. Erik Hack, Arlène K. van Vliet, Mercedes Valls Seron |
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| Přispěvatelé: | Molecular cell biology and Immunology, Surgery, Pathology, Internal medicine, Laboratory Medicine, Neurology, Landsteiner Laboratory, Amsterdam Gastroenterology Endocrinology Metabolism |
| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
Male
medicine.medical_specialty Neutrophils Phosphorylcholine Ischemia Capillary Permeability Internal medicine medicine Complement C4b Animals Intestinal Mucosa Rats Wistar Complement Activation biology business.industry C-reactive protein Albumin Acute-phase protein Complement C3 medicine.disease Immunohistochemistry Peptide Fragments Complement system Rats Intestines Disease Models Animal Endocrinology C-Reactive Protein Immunoglobulin M Reperfusion Injury Immunology biology.protein Surgery business Reperfusion injury |
| Zdroj: | Padilla, N D, van Vliet, A K, Schoots, I G, Valls Seron, M, Maas, M A, Peltenburg, E E P, de Vries, A, Niessen, H W M, Hack, C E & van Gulik, T M 2007, ' C-reactive protein and natural IgM antibodies are activators of complement in a rat model of intestinal ischemia and reperfusion ', Surgery, vol. 142, no. 5, pp. 722-33 . https://doi.org/10.1016/j.surg.2007.05.015 Surgery, 142(5), 722-33. Mosby Inc. Surgery, 142(5), 722-733. Mosby Inc. |
| ISSN: | 0039-6060 |
| Popis: | BACKGROUND: The role of C-reactive protein (CRP), natural immunoglobulin M (IgM), and natural IgM against phosphorylcholine (anti-Pc IgM) was investigated in relation with complement activation in a rat model of intestinal ischemia and reperfusion (II/R). The effect of C1-esterase inhibitor (C1-Inh) on this complement activation along with other inflammatory mediators was also studied.METHODS: Rats were subjected to 1 h of superior mesenteric artery occlusion and 3 h of reperfusion. Intravenous administration of vehicle (human albumin) or C1-Inh (200 U/kg) was performed before (n = 8) or after ischemia (n = 8). II/R increased levels of C4b/c, CRP, IgM, anti-Pc IgM, and myeloperoxidase activity in the intestinal homogenates and induced vascular leakage.RESULTS: A good correlation was observed in the intestinal homogenates between C4b/c and CRP levels. Clear depositions of C3, CRP, and IgM in intestinal tissue were demonstrated after II/R, and a strong correlation of both CRP and IgM with complement was observed. C1-Inh administered before ischemia reduced the complement activation response after II/R, as reflected by decreased levels of C4b/c in conjunction with reduced anti-Pc IgM in the intestinal homogenates. C1-Inh also decreased leakage of albumin when administered before ischemia. C1-Inh after ischemia reduced C4b/c levels and myeloperoxidase activity in the homogenates.CONCLUSIONS: CRP and IgM depositions correlated well with local complement activation, which suggests a role of these molecules in complement activation. Furthermore, C1-Inh inhibited potentially II/R injury either administered before or after ischemia, by attenuating complement activation induced by CRP and/or natural IgM antibodies. |
| Databáze: | OpenAIRE |
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