Substrate-dependent differential expression of Humicola grisea var. thermoidea cellobiohydrolase genes
| Autor: | Marcio José Poças-Fonseca, Ildinete Silva-Pereira, M O Azevedo, Bruno B Rocha |
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| Rok vydání: | 2000 |
| Předmět: |
Transcription
Genetic Immunology Catabolite repression Cellulase Applied Microbiology and Biotechnology Microbiology Lignin Substrate Specificity Gene product Ascomycota Transcription (biology) Gene Expression Regulation Fungal Gene expression Genetics Cellulose 1 4-beta-Cellobiosidase Cellulose Molecular Biology Gene chemistry.chemical_classification Regulation of gene expression biology Reverse Transcriptase Polymerase Chain Reaction General Medicine Carbon Culture Media Enzyme Biochemistry chemistry biology.protein |
| Zdroj: | Canadian journal of microbiology. 46(8) |
| ISSN: | 0008-4166 |
| Popis: | Transcription of fungal cellulase genes may be affected by substrate induction. We studied the expression of Humicola grisea var. thermoidea cellobiohydrolase genes (cbh1.1 and cbh1.2) under induction by several soluble and insoluble carbon sources. Using the RT-PCR technique, the cbh1.2 transcript was detected in all the conditions assayed along the growth curve. Catabolite repression, which frequently occurs in other fungal cellulolytic systems, was not observed. On the other hand, cbh1.1 transcription was shown to be driven by insoluble and complex lignocellulosic substrates. In summary, the cbh1.2 gene product is constitutively produced, while cbh1.1 seems to respond to a distinct regulatory mechanism.Key words: Humicola, cellobiohydrolase genes, gene expression, carbon sources, lignocellulosic substrates. |
| Databáze: | OpenAIRE |
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