Rotavirus-like particles: a novel nanocarrier for the gut
| Autor: | Naima G. Cortes-Perez, Philippe Seksik, Laurent Beaugerie, Philippe Langella, Germain Trugnan, Loïc Jaffrelo, Jean Pierre Grill, Catherine Sapin, Sabine Daou, Serge Chwetzoff |
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| Přispěvatelé: | Université Pierre et Marie Curie - Paris 6 (UPMC), Service de Gastroentérologie et nutrition [CHU Saint-Antoine], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-CHU Saint-Antoine [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Sorbonne Université (SU), Trafic Membranaire et Signalisation Dans les Cellules Epitheliales, Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM), CHU Saint-Antoine [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU) |
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Male
Rotavirus Health Toxicology and Mutagenesis viruses lcsh:Medicine medicine.disease_cause Green fluorescent protein Mice Drug Delivery Systems Intestinal mucosa Intestinal Mucosa Mice Inbred BALB C 0303 health sciences [CHIM.ORGA]Chemical Sciences/Organic chemistry 030302 biochemistry & molecular biology virus diseases General Medicine Colitis 3. Good health Capsid Drug delivery Molecular Medicine Baculoviridae Research Article Biotechnology Article Subject lcsh:Biotechnology Green Fluorescent Proteins Enzyme-Linked Immunosorbent Assay Spodoptera Biology Cell Line 03 medical and health sciences lcsh:TP248.13-248.65 Genetics medicine Animals Molecular Biology Tropism 030304 developmental biology Analysis of Variance lcsh:R Virion Virus Internalization Molecular biology In vitro Disease Models Animal Trinitrobenzenesulfonic Acid Cell culture appareil digestif Nanoparticles |
| Zdroj: | Journal of Biomedicine and Biotechnology Journal of Biomedicine and Biotechnology, Hindawi Publishing Corporation, 2010, 2010, pp.317545. ⟨10.1155/2010/317545⟩ Journal of Biomedicine and Biotechnology (2010), . (2010) Journal of Biomedicine and Biotechnology, Vol 2010 (2010) Journal of Biomedicine and Biotechnology, 2010, 2010, pp.317545. ⟨10.1155/2010/317545⟩ |
| ISSN: | 1110-7243 1110-7251 |
| Popis: | International audience; The delivery of bioactive molecules directly to damaged tissues represents a technological challenge. We propose here a new system based on virus-like particles (VLP) from rotavirus, with a marked tropism for the gut to deliver bio-active molecules to intestinal cells. For this, nonreplicative VLP nanoparticles were constructed using a baculovirus expression system and used to deliver an exogenous biomolecule, the green fluorescent protein (GFP), into either MA104 cells or intestinal cells from healthy and 2,4,6-trinitrobenzene sulfonic acid (TNBS)-treated mice. Our results show that expression of rotavirus capsid proteins in baculovirus led to the auto assembly of VLP that display similar properties to rotavirus. In vitro experiments showed that VLP were able to enter into MA104 cells and deliver the reporter protein. Intragastric administration of fluorescent VLP in healthy and TNBS-treated mice resulted in the detection of GFP and viral proteins in intestinal samples. Our results demonstrate an efficient entry of non-replicative rotavirus VLP into the epithelial cell line MA104 and provide the first in vivo evidence of the potential of these nanoparticles as a promising safe candidate for drug delivery to intestinal cells. |
| Databáze: | OpenAIRE |
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