Significance of microRNA-330-5p/TYMS Expression Axis in the Pathogenesis of Colorectal Tumorigenesis

Autor: Leila Karimi, Habib Zarredar, Saman Niknam, Venus Zafari, Milad Asadi, Soghra Bornehdeli, Milad Jaberi, Touraj Asvadi Kermani
Rok vydání: 2021
Předmět:
Carcinogenesis
Colorectal cancer
microRNA 330 5p
cell transformation
Thymidylate synthase
Pathogenesis
middle aged
genetics
Lymph node
RNA transcription
microRNA
lymph node metastasis
biology
alternative medicine
adult
Gastroenterology
gene expression regulation
Prognosis
cohort analysis
unclassified drug
Gene Expression Regulation
Neoplastic
female
Cell Transformation
Neoplastic
medicine.anatomical_structure
Oncology
real time polymerase chain reaction
cancer therapy
diagnostic value
protein RNA binding
Colorectal Neoplasms
down regulation
cancer tissue
TYMS protein
human
In silico
colorectal cancer
thymidylate synthase
TYMS
Article
MIRN330 microRNA
human
male
Cell Line
Tumor
medicine
Humans
controlled study
diagnostic test accuracy study
human
protein expression
Cell Proliferation
business.industry
Cell growth
cancer staging
tumor cell line
Cancer
medicine.disease
major clinical study
human tissue
miR-330-5p
MicroRNAs
gene expression
Cancer research
biology.protein
pathology
computer model
business
metabolism
upregulation
colorectal tumor
Zdroj: Journal of Gastrointestinal Cancer. 53:965-970
ISSN: 1941-6636
1941-6628
DOI: 10.1007/s12029-021-00695-x
Popis: Background: Colorectal cancer (CRC) is one of the most common types of cancer worldwide. A number of dysregulated microRNAs (miRNAs) have been linked to CRC progression and treatment response and are thought to be promising prognostic biomarkers for this cancer. microRNA-330 (miR-330-5p) has been reported to inhibit cell proliferation through suppressing thymidylate synthase (TYMS). In the current study, miR-330-5p, TYMS, and their interactions were investigated to evaluate their therapeutic and diagnostic value for CRC treatment. Methods: The expression levels of miR-330-5p and TYMS were evaluated in silico using TCGA datasets for CRC. Data validation was performed on a set of internal samples (100 pairs of CRC tumor specimens and adjacent non-cancerous samples) utilizing real-time PCR assay. The linkage between clinicopathological parameters and expression levels was also investigated. Results: TCGA results indicated that miR-330-5p and TYMS were significantly upregulated and downregulated in the CRC, respectively. Real-time PCR results confirmed that the expression of miR-330-5p was significantly upregulated in tumor tissues relative to marginal tissues (P = 0.0005), whereas TYMS expression was significantly downregulated (P = 0.0001). The transcript level of miR-330-5p was associated with tumor stage and lymph node metastases. Conclusion: The microRNA-330 inhibited cell proliferation by suppressing thymidylate synthase (TYMS) in colorectal cancer. Therefore, suggesting that they are valuable factors for further studies of alternative treatment and diagnostic methods. © 2021, Springer Science+Business Media, LLC, part of Springer Nature.
This study was supported by a grant from the research deputy of the Department of Tuberculosis and Lung Diseases Research Center, University Tabriz University of Medical Sciences, Tabriz, Iran.
Databáze: OpenAIRE
Externí odkaz: