HLA-DRB1 gene transcripts in rheumatoid arthritis
| Autor: | B. Combe, J. F. Eliaou, Sophie Kerlan-Candon, V. Pinet, J. Clot, R. Vincent |
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| Rok vydání: | 2001 |
| Předmět: |
musculoskeletal diseases
Genotype Genes MHC Class II Immunology Arthritis Autoimmune Diseases Arthritis Rheumatoid immune system diseases Gene expression medicine Humans Immunology and Allergy Genetic Predisposition to Disease RNA Messenger Allele skin and connective tissue diseases Gene HLA-DRB1 Alleles Regulation of gene expression MHC class II biology Reverse Transcriptase Polymerase Chain Reaction Immunity to Infection HLA-DR Antigens medicine.disease Gene Expression Regulation biology.protein Immunosuppressive Agents HLA-DRB1 Chains |
| Zdroj: | Clinical and Experimental Immunology. 124:142-149 |
| ISSN: | 1365-2249 0009-9104 |
| Popis: | SUMMARYSusceptibility to rheumatoid arthritis (RA) is associated with defined HLA-DRB1 alleles. However the molecular basis of this association is not known. Peculiarities in the expression of disease-linked DRB1 alleles could be involved since in healthy controls HLA-DRB1 gene expression varies according to the alleles in B cells. Peripheral blood B cells of healthy controls and RA patients were examined for their level of allelic DRB1 transcripts using a competitive PCR approach. Levels of DRB1 transcripts were greatly modified in RA and influenced by HLA-DRB1 genotype: patients with double dose of RA-associated alleles displayed up-regulated amounts of DRB1 gene transcripts whereas patients carrying either a single or no at risk allele had low levels of DRB1 transcripts, compared to control individuals. These differential levels of DRB1 gene expression were not influenced in any way by clinical, biological or therapeutic features of the patients. Various amounts of DRB1 mRNA may be related to variations of the density of DR molecules on B cells and consequently could influence the response of CD4 T cells. This particular regulation of DRB1 gene expression in RA patients could therefore represent one of the molecular mechanisms involved in the association of HLA DRB1 genes to RA. |
| Databáze: | OpenAIRE |
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