DNA Probe for Lactobacillus delbrueckii
| Autor: | Beat Mollet, Michèle Delley, Herbert Hottinger |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
clone (Java method)
Ecology Auxotrophy Hybridization probe food and beverages Genetics and Molecular Biology Biology Molecular cloning medicine.disease_cause biology.organism_classification Applied Microbiology and Biotechnology Molecular biology chemistry.chemical_compound genomic DNA chemistry Biochemistry Lactobacillus medicine Escherichia coli DNA Food Science Biotechnology |
| Zdroj: | Applied and environmental microbiology. 56(6) |
| ISSN: | 0099-2240 |
| Popis: | From a genomic DNA library of Lactobacillus delbrueckii subsp. bulgaricus, a clone was isolated which complements a leucine auxotrophy of an Escherichia coli strain (GE891). Subsequent analysis of the clone indicated that it could serve as a specific DNA probe. Dot-blot hybridizations with over 40 different Lactobacillus strains showed that this clone specifically recognizes L. delbrueckii subsp. delbrueckii, bulgaricus, and lactis. The sensitivity of the method was tested by using an α- 32 P-labeled DNA probe. |
| Databáze: | OpenAIRE |
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