Collection of allogeneic peripheral blood progenitor cells by two protocols on an apheresis system
Autor: | H Büchele, Thomas Schreiner, S. De Reys, J Kalinova, E Krug, E Erne, B Maccari, Bischof C, Markus Wiesneth |
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Rok vydání: | 2008 |
Předmět: |
Adult
Male medicine.medical_specialty Time Factors Allogeneic transplantation Immunology Urology Antigens CD34 Blood Donors Cell Count Leukocyte Count Humans Transplantation Homologous Immunology and Allergy Medicine Platelet Progenitor cell Platelet Count business.industry Hematology Venous blood Middle Aged Hematopoietic Stem Cells Granulocyte colony-stimulating factor Surgery Transplantation Apheresis medicine.anatomical_structure Blood Component Removal Female Bone marrow business |
Zdroj: | Transfusion. 38:1051-1055 |
ISSN: | 1537-2995 0041-1132 |
Popis: | BACKGROUND: Granulocyte-colony stimulating factor-mobilized allogeneic peripheral blood progenitor cells (PBPCs) are replacing bone marrow in transplantation for the treatment of several hematologic malignancies. The advantages of PBPCs are offset by the donor-associated disadvantages of granulocyte-colony stimulating factor side effects and the risk of apheresis-like platelet loss. STUDY DESIGN AND METHODS: For each individual, the first donation of allogeneic PBPCs by apheresis on the Spectra, using either the standard protocol Version 4.7 (45 donors, [Version 4.7]) or the AutoPBSC (60 donors, [AutoPBSC]) was compared. Between July 1995 and May 1996, all donors enrolled underwent Version 4.7 apheresis. Since May 1996, the majority of donors underwent AutoPBSC apheresis. For statistical analysis, only data from the first apheresis for each individual donor was considered for independent values. RESULTS: These results indicate a similar collection efficiency for CD34+ cells in the first apheresis of each donor (54% Version 4.7 vs. 53% AutoPBSC, p = 0.8). The apheresis time was longer with the AutoPBSC (233 min vs. 251 min, p = 0.005), whereas the loss of platelets was significantly lower (p < 0.001) with the AutoPBSC (28% vs. 19%). The mean number of CD34+ cells collected in the first apheresis component was 4.0 × 10(8) (Version 4.7) versus 3.8 × 10(8) (AutoPBSC). CONCLUSION: Both apheresis protocols collect sufficient numbers of PBSCs for allogeneic transplantation. The AutoPBSC operates in a fully automatic fashion, avoiding manual adjustment and interindividual variations. The loss of platelets is lower with AutoPBSC than with Version 4.7, but the apheresis time is slightly longer. |
Databáze: | OpenAIRE |
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