Estramustine: A novel radiation enhancer in human carcinoma cells
| Autor: | Jae Ho Kim, Molly Gabel, Mark S. Khil, Yong J. Lee, Samuel Ryu, Sang-Hie Kim |
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| Rok vydání: | 1994 |
| Předmět: |
Male
Radiation-Sensitizing Agents Cancer Research Pathology medicine.medical_specialty Paclitaxel Cell Survival Breast Neoplasms Vinblastine Microtubules Flow cytometry HeLa Neoplasms Tumor Cells Cultured medicine Carcinoma Humans Radiology Nuclear Medicine and imaging Radiosensitivity Radiation biology medicine.diagnostic_test Antimicrotubule agent business.industry Cell Cycle Prostatic Neoplasms Glioma Cell cycle Flow Cytometry medicine.disease biology.organism_classification Combined Modality Therapy Oncology Cell culture Colonic Neoplasms Estramustine Cancer research Female business HeLa Cells medicine.drug |
| Zdroj: | International Journal of Radiation Oncology*Biology*Physics. 30:99-104 |
| ISSN: | 0360-3016 |
| DOI: | 10.1016/0360-3016(94)90524-x |
| Popis: | Purpose: Estramustine (EM), an antimicrotubule agent, binds microtubule-associated proteins, causes spindle dis-assembly, and arrests cells at the late G 2 /M phase of the cell cycle. Since cells in the G 2 /M phase are the most radiosensitive and some human cancer cells contain high level of EM-binding protein, experiments were carried out to determine whether radiation sensitization could be obtained in human carcinoma cells. Methods and Materials: Cells containing a high level of EM-binding protein such as prostate carcinoma (DU-145), breast carcinoma (MCF-7), and malignant glioma (U-251) were used to demonstrate radiosensitization. Cervical carcinoma (HeLa-S 3 ) and colon carcinoma (HT-29) cells which are not known to contain EM-binding protein were also employed. Cell survival was assayed by the colony forming ability of single plated cells in culture to obtain dose-survival curves. Results: Pretreatment of DU-145, MCF-7, and U-251 cells to a nontoxic concentration (5 μM) of EM for more than one cell cycle time, substantially enhanced the radiation-induced cytotoxicity. The sensitizer enhancement ratio of these cells ranged from 1.35–1.52. The magnitude of the enhancement was dependent on the drug concentration and exposure time. The rate of cell accumulation in G 2 /M phase, as determined by flow cytometry, increased with longer treatment time in the cell lines which showed radiosensitization. Other antimicrotubule agents such as taxol and vinblastine caused minimal or no radiosensitization at nontoxic concentrations. Conclusion: The data provide a radiobiological basis for using EM as a novel radiation enhancer, with the property of tissue selectivity. |
| Databáze: | OpenAIRE |
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