Stimulation of cell proliferation by glutathione monoethyl ester in aged bone marrow stromal cells is associated with the assistance of TERT gene expression and telomerase activity
| Autor: | Seyed Alireza Mesbah-Namin, Taki Tiraihi, Taher Taheri, Najmeh Aminizadeh |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Telomerase Stromal cell Cellular differentiation Telomere-Binding Proteins Bone Marrow Cells Biology Choline O-Acetyltransferase 03 medical and health sciences medicine Animals Humans Telomerase reverse transcriptase Cell Proliferation Telomere-binding protein Neurons Gene Expression Regulation Developmental Cell Differentiation Mesenchymal Stem Cells Cell Biology General Medicine Telomere Molecular biology Glutathione Rats 030104 developmental biology medicine.anatomical_structure Cell culture Bone marrow Developmental Biology |
| Zdroj: | In vitro cellulardevelopmental biology. Animal. 52(7) |
| ISSN: | 1543-706X |
| Popis: | The proliferation and differentiation potential of aged bone marrow stromal cells (BMSCs) are significantly reduced. In order to improve the performance of the aged BMSCs, these cells were treated with 2 mM glutathione monoethyl ester (GSH-MEE) for 24 h. Proliferation rate, telomerase activity, telomere length, and differentiation to cholinergic neuron-like cells (CNLCs) were observed to increase. Though, the expression level of telomerase reverse transcriptase gene increased, but CTC1 and TEN1 genes from Ctc1-Stn1-Ten1 complex encoding proteins with regulatory function significantly decreased. Trypan blue exclusion assay was used to analyze the proliferation and, while telomere length, its several related gene expressions, and telomerase activity were measured using the real time reverse transcription-polymerase chain reaction and polymerase chain reaction enzyme-linked immunosorbent assay techniques, respectively. CNLCs differentiation potential was evaluated by estimating the percentage of choline acetyltransferase immunereactive cells.The results suggested that GSH-MEE could improve aged rat BMSC properties and would be of potential benefit for enhancing the performance of aged people's BMSCs. |
| Databáze: | OpenAIRE |
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