Differences in TLR9-dependent inhibitory effects of H2O2-induced IL-8 secretion and NF-kappa B/I kappa B-alpha system activation by genomic DNA from five Lactobacillus species
| Autor: | Nobuhiro Kashige, Shota Shiimura, Keiichi Irie, Yuki Fukumitsu, Yukihiko Nakashima, Tomomitsu Satho, Saori Uyeda, Fumio Miake, Yukihiro Hiramatsu, Tanjina Sharmin, Takahiro Okuno |
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| Rok vydání: | 2013 |
| Předmět: |
DNA
Bacterial Lactobacillus casei Immunology Active Transport Cell Nucleus Anti-Inflammatory Agents Lactobacillus gasseri Microbiology NF-KappaB Inhibitor alpha Lactobacillus Escherichia coli Humans Secretion Receptor Cell Nucleus Dose-Response Relationship Drug biology Interleukin-8 NF-kappa B food and beverages Hydrogen Peroxide biology.organism_classification Molecular biology genomic DNA Infectious Diseases Toll-Like Receptor 9 Proteolysis I-kappa B Proteins RNA Interference Caco-2 Cells Genome Bacterial Bacteria Lactobacillus plantarum Signal Transduction |
| Zdroj: | Microbes and Infection. 15:96-104 |
| ISSN: | 1286-4579 |
| Popis: | Lactic acid bacteria (LAB) show anti-inflammatory effects, and their genomic DNA was identified as one of the anti-inflammatory components. Despite the differences in anti-inflammatory effects between live LAB dependent not only on genus but also species, this effect has not been compared at the genomic DNA level. We compared the anti-inflammatory effects of the genomic DNA from five Lactobacillus species-Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus gasseri, Lactobacillus plantarum, and Lactobacillus reuteri-using Caco-2 cells. To evaluate anti-inflammatory effects, decreases in H(2)O(2)-induced IL-8 secretion and inhibition of H(2)O(2)-induced NF-κB/IκB-α system activation were examined. All LAB genomic DNAs dose-dependently decreased H(2)O(2)-induced IL-8 secretion and inhibited H(2)O(2)-induced NF-κB/IκB-α system activation. Comparison of these effects between Lactobacillus species showed that the anti-inflammatory effects of L. acidophilus genomic DNA are lower than those of the other species. Furthermore, suppression of Toll-like receptor 9 (TLR9), a specific receptor of bacterial DNA, expression by RNAi abolished the decrease of H(2)O(2)-induced IL-8 secretion and inhibition of H(2)O(2)-induced NF-κB/IκB-α system activation by LAB genomic DNA. Our results demonstrated that the anti-inflammatory effects of genomic DNA differ between Lactobacillus species and TLR9 is one of the major pathways responsible for the anti-inflammatory effect of LAB genomic DNA. |
| Databáze: | OpenAIRE |
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