Regulation of lipogenic capacity in lactating rats
| Autor: | Arie Geursen, M. J. Sneyd, Murray R. Grigor, S M Warren |
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| Rok vydání: | 1982 |
| Předmět: |
History
medicine.medical_specialty Litter Size Mammary gland Dehydrogenase Biology Malate dehydrogenase Education chemistry.chemical_compound Mammary Glands Animal Pregnancy Internal medicine Lactation Lactate dehydrogenase medicine Animals chemistry.chemical_classification Rats Inbred Strains Dietary Fats Lipids Rats Computer Science Applications Fatty acid synthase medicine.anatomical_structure Endocrinology Enzyme Liver chemistry Lipogenesis biology.protein Female Research Article |
| Zdroj: | Biochemical Journal. 208:611-618 |
| ISSN: | 0306-3283 |
| DOI: | 10.1042/bj2080611 |
| Popis: | 1. The rate of mammary-gland lipogenesis measured in vivo from 3H2O was suppressed after decreasing the milk demand by decreasing the number of pups from ten to two or three, as well as by giving diets containing lipid [Grigor & Warren (1980) Biochem. J. 188, 61-65]. 2. The specific activities of the lipogenic enzymes fatty acid synthase, glucose 6-phosphate dehydrogenase and ‘malic’ enzyme increased between 6- and 10-fold in the mammary gland and between 2- and 3-fold in the livers during the first 10 days of lactation. The increases in specific activity coupled with the doubling of liver mass which occurred during pregnancy and lactation resulted in considerable differences in total liver activities when compared with virgin animals. 3. Although consumption of a diet containing 20% peanut oil suppressed the activities of the three lipogenic enzymes in the livers, only the ‘malic’ enzyme was affected in the mammary glands. 4. In contrast, decreased milk demand did not affect the specific activities of any of the liver enzymes, whereas it resulted in suppression of all three lipogenic enzymes of the mammary glands. There was no effect on either the cytoplasmic malate dehydrogenase or the lactate dehydrogenase of the mammary gland. 5. In all the experiments performed, the activity of the fatty acid synthase correlated with the amount of material precipitated by the rabbit antibody raised against rat fatty acid synthase. |
| Databáze: | OpenAIRE |
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