Variations in the Bioactive Compounds Composition and Biological Activities of Loofah (Luffa cylindrica) Fruits in Relation to Maturation Stages
| Autor: | Feten Belhadj, Fatih Gul, M. Nejib Marzouki, Ayse Sahin Yaglioglu, Ibrahim Demirtas, Takoua Ben Hlel, Muhammed Altun |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Antioxidant medicine.medical_treatment Bioengineering Biochemistry Antioxidants HeLa 03 medical and health sciences chemistry.chemical_compound Structure-Activity Relationship 0302 clinical medicine medicine Tumor Cells Cultured Humans Food science Cellulose Cytotoxicity Medicinal plants Molecular Biology Cell Proliferation biology Dose-Response Relationship Drug Cell growth General Chemistry General Medicine Free Radical Scavengers biology.organism_classification Antineoplastic Agents Phytogenic 030104 developmental biology chemistry Cell culture 030220 oncology & carcinogenesis Fruit Molecular Medicine Composition (visual arts) Drug Screening Assays Antitumor Luffa HeLa Cells |
| Zdroj: | Chemistrybiodiversity. 14(10) |
| ISSN: | 1612-1880 |
| Popis: | The present work aimed to determine the antioxidant and antiproliferative potential of Luffa cylindrica fruits collected at two different maturation stages and to identify and compare their functional components composition. The MeOH extracts of L. cylindrica fruits harvested at 60 - 65 days after seeding (S1) and 85 - 90 days after seeding (S2) were investigated for their antioxidant activity using various assays. Furthermore, the antiproliferative activity of the extracts against HeLa human cervical cancer cells was explored with xCELLigence real time cell analyzer, while the effect of the samples on the membrane integrity of the same cell line was assessed using LDH cytotoxicity leakage assay. Ultimately, the phytochemicals were analyzed using GC/MS and HPLC/TOF-MS. The S1 sample had higher contents and more diversity in the phenolic compounds composition than S2. Furthermore, the S1 extract showed the highest antioxidant and antiproliferative activity, while the S2 extract had higher cytotoxicity towards HeLa cells. The findings revealed that the time of harvest has a big impact on the phytochemicals content and activity and that harvesting L. cylindrica at an early stage before the beginning of the development of the cellulose fibrous system is recommended for a rich phytochemical composition and efficient antioxidant and antiproliferative activities. |
| Databáze: | OpenAIRE |
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