Effect of Co-exposure to Heat and Psychological Stressors on Sperm DNA and Semen Parameters
| Autor: | Abbas Haghparast, Siamak Sabour, Somayeh Farhang Dehghan, Rezvan Zendehdel, Farnaz Abdollahi, Saeid Amanpour |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Heat Stress
DNA damage Health Toxicology and Mutagenesis Motility Psychological Stress Semen AO Acridine Orange Toxicology Andrology Semen quality RA1190-1270 Medicine THI Temperature Humidity Index Co-exposure CASA Computer-Assisted Sperm Analysis ComputingMethodologies_COMPUTERGRAPHICS business.industry ANOVA Analysis OF Variance Sperm DNA Stressor Sperm dna Regular Article Sperm CPCSEA Committee for the Purpose Of Control and Supervision Of Experiments on Animals Toxicology. Poisons Rat BSA Bovine Serum Albumin Co exposure business DNA Deoxyribonucleic Acid ROS Reactive Oxygen Species |
| Zdroj: | Toxicology Reports Toxicology Reports, Vol 8, Iss, Pp 1948-1954 (2021) |
| ISSN: | 2214-7500 |
| Popis: | Graphical abstract Highlights • Co-exposure to heat and psychological stressors on semen quality have been studied. • Combined exposure group had significantly lower semen quality compared with those of others. • Heat exposure group had a higher percentage of sperm DNA damage compared to others. The present study aims to investigate the effects of co-exposure to heat and psychological stress on sperm DNA and semen parameters among male rats. The study was conducted on 40 healthy adult male Wistar rats. The rats were randomly categorized into four groups of same size consisting of a control group, a heat stress, psychological and co-exposure groups. The heat stress group was exposed to a temperature of 36 °C at 20% relative humidity. The psychological stress exposure group was subjected to three stressors including exposure to strobe light, noise and tilting cage. According the results,the co-exposure group had lower mean sperm parameters including sperm count (17.22 ± 4.22 106/ml), motility (42.63 ± 12.95 %), viability (48.50 ± 23.25 %), normal morphology (56 ± 7.5%), progressive motility (11.61 ± 7.81%), non-progressive motility (31.18 ± 7.77%), curvilinear velocity (24.11 ± 3.81 μm/s) and straight-line velocity (3.2 ± 1.4 μm/s) when compared with those of the other groups (P = 0.001). Mean sperm immobility (57.36 ± 12.95%) and non-progressive motility (37.93 ± 11.15%) in the co-exposure group was higher compared to the other groups (P = 0.001 and P = 0.333, respectively). Assessment of damage to sperm DNA revealed that the heat exposure group had a higher percentage of sperm DNA damage (9.44 ± 6.80 %) compared to others (P = 0.185). In case of all of exposure scenario, the chance that the semen quality decreased compared to the control group has been increased. In general the combined stress had a greater significant effect on sperm parameters compared to other exposure groups, except for DNA damage. |
| Databáze: | OpenAIRE |
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