Experimental Methods for Determining Permethrin Dermal Absorption
| Autor: | Jeffrey H. Driver, William G. Reifenrath, John H. Ross |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Male
Piperonyl butoxide Piperonyl Butoxide Skin Absorption Health Toxicology and Mutagenesis Urine Absorption (skin) In Vitro Techniques Pharmacology Toxicology Models Biological Rats Sprague-Dawley Feces Random Allocation chemistry.chemical_compound In vivo parasitic diseases medicine Animals Humans Tissue Distribution Carbon Radioisotopes Pesticides Permethrin Skin Ethanol Chromatography Dose-Response Relationship Drug Chemistry Middle Aged In vitro Rats Female Experimental methods Environmental Health Algorithms medicine.drug |
| Zdroj: | Journal of Toxicology and Environmental Health, Part A. 74:325-335 |
| ISSN: | 1087-2620 1528-7394 |
| Popis: | The objectives of this study were to (1) determine the percutaneous absorption of radiolabeled permethrin and piperonyl butoxide (PBO) in vivo in rats and in vitro to permit a calculation of the ratio of in vitro to in vivo values, and (2) test a method of estimating in vivo human absorption. Carbon-14 labeled permethrin in ethanol solution was applied to the clipped skin of rats in vivo at doses of 2.25, 20, or 200 μg/cm2. As a reference compound, 14C-labeled PBO in isopropanol solution was applied to rat skin in vivo at a dose of 100 μg/cm2. All applications were washed at 24 h postapplication, and rats were sacrificed either at 24 h for permethrin or 5 d for both compounds. The radiolabel recovered from carcass, urine including cage wash, and feces was summed to determine percent absorption. For the 24-h time point, at doses of 2.25, 20, and 200 μg/cm2 of permethrin, values of 22, 22, and 28%, respectively, were obtained for in vivo rat percutaneous absorption (n=6 per dose). For the 5-d time point, at doses of 2.25, 20, and 200 μg/cm2 of permethrin, values of 38, 38, and 30%, respectively, were obtained for in vivo rat percutaneous absorption (n=6 per dose). The 5-d percutaneous absorption of 14C-PBO at 100 μg/cm2 was determined to be 42% (n=6). Dose and test duration did not exert a statistically significant effect on percutaneous absorption of permethrin in the rat in vivo. For in vitro absorption determination, 14C-permethrin in ethanol solution was applied to freshly excised human skin in an in vitro test system predictive of skin absorption in humans. Twenty-four hours after application, the radiolabel recovered from dermis and receptor fluid was summed to determine percent absorption. At doses of approximately 2.25, 20, and 200 μg/cm2 permethrin, values of 1, 3, and 2%, respectively, were obtained for percutaneous absorption (n=9 per dose). Excised human skin absorption of 14C-PBO at 100 μg/cm2 was determined to be 7% (n=9). Excised rat skin absorptions of permethrin at 2.25, 20, and 200 μg/cm2 were found to be 20, 18, and 24%, respectively (n=6 per dose), approximately 10-fold higher than human skin absorption. Excised rat skin absorption of PBO was also higher (35%) than the value obtained for human skin by a factor of about 5. |
| Databáze: | OpenAIRE |
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