Growth of endothelial cells in space and in simulated microgravity : a comparison on the secretory level
| Autor: | Thomas J. Corydon, Matthias Evert, Markus Wehland, Marcel Egli, Stefano Nebuloni, Jessica Pietsch, Johann Bauer, Daniel Grimm, Daniel T O Carvalho, Sara Dam Kobberø, Daniela Melnik, Manfred Infanger, Marjan Moreels, Marcus Krüger, Sarah Baatout, Samuel Gass, Sahana Jayashree, Sascha Kopp |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Physiology Spheroids Cellular/cytology Cell morphology Spaceflight lcsh:Physiology Epithelial Cells/cytology law.invention Cell Line lcsh:Biochemistry Extracellular matrix 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine law Spheroids Cellular Medicine and Health Sciences Humans lcsh:QD415-436 lcsh:QP1-981 Random positioning machine Weightlessness Spheroid Biology and Life Sciences Epithelial Cells Space Flight Cell biology Vascular endothelial growth factor 030104 developmental biology chemistry Gene Expression Regulation Cell culture 030220 oncology & carcinogenesis Cytokines Microgravity Spheroids |
| Zdroj: | CELLULAR PHYSIOLOGY AND BIOCHEMISTRY Krüger, M, Pietsch, J, Bauer, J, Kopp, S, Carvalho, D T O, Baatout, S, Moreels, M, Melnik, D, Wehland, M, Egli, M, Jayashree, S, Kobberø, S D, Corydon, T J, Nebuloni, S, Gass, S, Evert, M, Infanger, M & Grimm, D 2019, ' Growth of Endothelial Cells in Space and in Simulated Microgravity-a Comparison on the Secretory Level ', Cellular Physiology and Biochemistry, vol. 52, no. 5, pp. 1039-1060 . https://doi.org/10.33594/000000071 Cellular Physiology and Biochemistry, Vol 52, Iss 5, Pp 1039-1060 (2019) |
| ISSN: | 1015-8987 1421-9778 |
| DOI: | 10.33594/000000071 |
| Popis: | BACKGROUND/AIMS: Endothelial cells exposed to the Random Positioning Machine (RPM) reveal three different phenotypes. They grow as a two-dimensional monolayer and form three-dimensional (3D) structures such as spheroids and tubular constructs. As part of the ESA-SPHEROIDS project we want to understand how endothelial cells (ECs) react and adapt to long-term microgravity. METHODS: During a spaceflight to the International Space Station (ISS) and a subsequent stay onboard, human ECs (EA.hy926 cell line) were cultured for 12 days in real microgravity inside an automatic flight hardware, specially designed for use in space. ECs were cultivated in the absence or presence of vascular endothelial growth factor, which had demonstrated a cell-protective effect on ECs exposed to an RPM simulating microgravity. After cell fixation in space and return of the samples, we examined cell morphology and analyzed supernatants by Multianalyte Profiling technology. RESULTS: The fixed samples comprised 3D multicellular spheroids and tube-like structures in addition to monolayer cells, which are exclusively observed during growth under Earth gravity (1g). Within the 3D aggregates we detected enhanced collagen and laminin. The supernatant analysis unveiled alterations in secretion of several growth factors, cytokines, and extracellular matrix components as compared to cells cultivated at 1g or on the RPM. This confirmed an influence of gravity on interacting key proteins and genes and demonstrated a flight hardware impact on the endothelial secretome. CONCLUSION: Since formation of tube-like aggregates was observed only on the RPM and during spaceflight, we conclude that microgravity may be the major cause for ECs' 3D aggregation. ispartof: Cell Physiol Biochem vol:52 issue:5 pages:1039-1060 ispartof: location:Germany status: published |
| Databáze: | OpenAIRE |
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