Analysis of cyclophosphamide and five metabolites from human plasma using liquid chromatography–mass spectrometry and gas chromatography–nitrogen–phosphorus detection
| Autor: | Thomas F. Kalhorn, John T. Slattery, William N. Howald, Ross F. Lawrence, Song Ren |
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| Rok vydání: | 1999 |
| Předmět: |
Chromatography
Gas Chromatography Nitrogen Metabolite Phosphorus General Chemistry Mass spectrometry High-performance liquid chromatography Mass Spectrometry Standard curve chemistry.chemical_compound chemistry Liquid chromatography–mass spectrometry Humans Gas chromatography Derivatization Antineoplastic Agents Alkylating Cyclophosphamide Quantitative analysis (chemistry) Chromatography Liquid |
| Zdroj: | Journal of Chromatography B: Biomedical Sciences and Applications. 732:287-298 |
| ISSN: | 0378-4347 |
| DOI: | 10.1016/s0378-4347(99)00300-x |
| Popis: | An assay method for the quantification of cyclophosphamide (CY) and five metabolites from human plasma is presented. The procedure is adapted to the chemical properties of the compounds of interest: non-polar compounds are extracted into methylene chloride, concentrated and analyzed by GC-NPD after derivatization, and the remaining aqueous fraction is deproteinated with acetonitrile-methanol prior to separation via reversed-phase HPLC and detection using atmospheric pressure ionization (API)-MS. Standard curves are linear over the required range and reproducible over five months. Plasma concentration-time profiles of CY and metabolites from a patient receiving CY by intravenous infusion (60 mg/kg, once a day for two days) are presented. |
| Databáze: | OpenAIRE |
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