Particulate multivalent presentation of the receptor binding domain induces protective immune responses against MERS-CoV

Autor: Okba, Nisreen M A, Widjaja, Ivy, van Dieren, Brenda, Aebischer, Andrea, van Amerongen, Geert, de Waal, Leon, Stittelaar, Koert J, Schipper, Debby, Martina, Byron, van den Brand, Judith M A, Beer, Martin, Bosch, Berend-Jan, Haagmans, Bart L, LS Virologie, VPDC pathologie, dPB I&I, dPB CR, Virologie, dI&I I&I-1
Přispěvatelé: Virology, LS Virologie, VPDC pathologie, dPB I&I, dPB CR, Virologie, dI&I I&I-1
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0301 basic medicine
Epidemiology
viruses
Antibody Affinity
medicine.disease_cause
Antibodies
Viral

Virus Replication
Immunogenicity
Vaccine

Middle East Respiratory Syndrome Coronavirus/immunology
Drug Discovery
Antibodies
Viral/immunology

Viral/immunology
Antigen Presentation
Chemistry
Immunogenicity
General Medicine
Articles
Antibodies
Neutralizing/immunology

Spike Glycoprotein
Vaccination
Infectious Diseases
Spike Glycoprotein
Coronavirus

Spike Glycoprotein
Coronavirus/biosynthesis

Middle East Respiratory Syndrome Coronavirus
Female
Rabbits
Coronavirus Infections
Research Article
Protein Binding
spytag-spycatcher
Middle East respiratory syndrome coronavirus
030106 microbiology
Antigen presentation
Immunology
Genetic Vectors
rabbit
Enzyme-Linked Immunosorbent Assay
Microbiology
Antibodies
Coronavirus Infections/immunology
03 medical and health sciences
Immune system
Protein Domains
MERS-coronavirus
Neutralization Tests
Virology
medicine
Animals
Humans
Avidity
lumazine synthase
Binding Sites
SARS-CoV-2
Viral Vaccines
spike
biochemical phenomena
metabolism
and nutrition

Antibodies
Neutralizing

Neutralizing/immunology
Heptad repeat
030104 developmental biology
HEK293 Cells
Viral Vaccines/immunology
Viral replication
i301
Antibody Formation
Parasitology
Coronavirus/biosynthesis
Vaccine
Zdroj: Emerging Microbes & Infections
article-version (VoR) Version of Record
Emerging Microbes & Infections, 9(1), 1080-1091. Nature Publishing Group
Emerging Microbes and Infections, 9(1), 1080. Nature Publishing Group
ISSN: 2222-1751
Popis: Middle East respiratory syndrome coronavirus (MERS-CoV) is a WHO priority pathogen for which vaccines are urgently needed. Using an immune-focusing approach, we created self-assembling particles multivalently displaying critical regions of the MERS-CoV spike protein ─fusion peptide, heptad repeat 2, and receptor binding domain (RBD) ─ and tested their immunogenicity and protective capacity in rabbits. Using a “plug-and-display” SpyTag/SpyCatcher system, we coupled RBD to lumazine synthase (LS) particles producing multimeric RBD-presenting particles (RBD-LS). RBD-LS vaccination induced antibody responses of high magnitude and quality (avidity, MERS-CoV neutralizing capacity, and mucosal immunity) with cross-clade neutralization. The antibody responses were associated with blocking viral replication and upper and lower respiratory tract protection against MERS-CoV infection in rabbits. This arrayed multivalent presentation of the viral RBD using the antigen-SpyTag/LS-SpyCatcher is a promising MERS-CoV vaccine candidate and this platform may be applied for the rapid development of vaccines against other emerging viruses such as SARS-CoV-2.
Databáze: OpenAIRE