Particulate multivalent presentation of the receptor binding domain induces protective immune responses against MERS-CoV
Autor: | Okba, Nisreen M A, Widjaja, Ivy, van Dieren, Brenda, Aebischer, Andrea, van Amerongen, Geert, de Waal, Leon, Stittelaar, Koert J, Schipper, Debby, Martina, Byron, van den Brand, Judith M A, Beer, Martin, Bosch, Berend-Jan, Haagmans, Bart L, LS Virologie, VPDC pathologie, dPB I&I, dPB CR, Virologie, dI&I I&I-1 |
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Přispěvatelé: | Virology, LS Virologie, VPDC pathologie, dPB I&I, dPB CR, Virologie, dI&I I&I-1 |
Jazyk: | angličtina |
Rok vydání: | 2020 |
Předmět: |
0301 basic medicine
Epidemiology viruses Antibody Affinity medicine.disease_cause Antibodies Viral Virus Replication Immunogenicity Vaccine Middle East Respiratory Syndrome Coronavirus/immunology Drug Discovery Antibodies Viral/immunology Viral/immunology Antigen Presentation Chemistry Immunogenicity General Medicine Articles Antibodies Neutralizing/immunology Spike Glycoprotein Vaccination Infectious Diseases Spike Glycoprotein Coronavirus Spike Glycoprotein Coronavirus/biosynthesis Middle East Respiratory Syndrome Coronavirus Female Rabbits Coronavirus Infections Research Article Protein Binding spytag-spycatcher Middle East respiratory syndrome coronavirus 030106 microbiology Antigen presentation Immunology Genetic Vectors rabbit Enzyme-Linked Immunosorbent Assay Microbiology Antibodies Coronavirus Infections/immunology 03 medical and health sciences Immune system Protein Domains MERS-coronavirus Neutralization Tests Virology medicine Animals Humans Avidity lumazine synthase Binding Sites SARS-CoV-2 Viral Vaccines spike biochemical phenomena metabolism and nutrition Antibodies Neutralizing Neutralizing/immunology Heptad repeat 030104 developmental biology HEK293 Cells Viral Vaccines/immunology Viral replication i301 Antibody Formation Parasitology Coronavirus/biosynthesis Vaccine |
Zdroj: | Emerging Microbes & Infections article-version (VoR) Version of Record Emerging Microbes & Infections, 9(1), 1080-1091. Nature Publishing Group Emerging Microbes and Infections, 9(1), 1080. Nature Publishing Group |
ISSN: | 2222-1751 |
Popis: | Middle East respiratory syndrome coronavirus (MERS-CoV) is a WHO priority pathogen for which vaccines are urgently needed. Using an immune-focusing approach, we created self-assembling particles multivalently displaying critical regions of the MERS-CoV spike protein ─fusion peptide, heptad repeat 2, and receptor binding domain (RBD) ─ and tested their immunogenicity and protective capacity in rabbits. Using a “plug-and-display” SpyTag/SpyCatcher system, we coupled RBD to lumazine synthase (LS) particles producing multimeric RBD-presenting particles (RBD-LS). RBD-LS vaccination induced antibody responses of high magnitude and quality (avidity, MERS-CoV neutralizing capacity, and mucosal immunity) with cross-clade neutralization. The antibody responses were associated with blocking viral replication and upper and lower respiratory tract protection against MERS-CoV infection in rabbits. This arrayed multivalent presentation of the viral RBD using the antigen-SpyTag/LS-SpyCatcher is a promising MERS-CoV vaccine candidate and this platform may be applied for the rapid development of vaccines against other emerging viruses such as SARS-CoV-2. |
Databáze: | OpenAIRE |
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