SIRT1 decelerates morphological processing of oligodendrocyte cell lines and regulates the expression of cytoskeleton-related oligodendrocyte proteins
| Autor: | Kazuki Yokokawa, Akihiro Matsumura, Tatsuo Manabe, Syuuichirou Suzuki, Shun Shimohama, Shin Hisahara, Mai Fujikura, Takashi Matsushita, Taro Saito, Yoshiyuki Horio, Naotoshi Iwahara, Jun Kawamata |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
animal structures endocrine system diseases Biophysics Nerve Tissue Proteins Biology SIRT2 Biochemistry Cell Line 03 medical and health sciences 0302 clinical medicine Sirtuin 2 Sirtuin 1 Tubulin Precursor cell medicine Humans Progenitor cell RNA Small Interfering Cytoskeleton Molecular Biology Cell Shape Acetylation Cell Differentiation Cell Biology Transfection Oligodendrocyte Cell biology enzymes and coenzymes (carbohydrates) Oligodendroglia 030104 developmental biology medicine.anatomical_structure Gene Expression Regulation Cell culture 030220 oncology & carcinogenesis Myelinogenesis biological phenomena cell phenomena and immunity hormones hormone substitutes and hormone antagonists |
| Zdroj: | Biochemical and biophysical research communications. 546 |
| ISSN: | 1090-2104 |
| Popis: | SIRT1 is involved in the regulation of a variety of biological processes such as metabolism, stress response, autophagy and differentiation. Although progenitor cells of oligodendrocytes (OPCs) express high level of SIRT1, its function on differentiation is unknown. Because we have shown that SIRT1 plays a pivotal role in differentiation of neural precursor cells, we hypothesized that SIRT1 may also participate in the differentiation of oligodendrocytes (OLGs). We examined whether SIRT1 was expressed in two human oligodendrocyte cell lines: KG-1-C and MO 3.13 OLG. Transfection of cell lines with SIRT1-siRNA and SIRT2-siRNA promoted the extension of cellular processes. SIRT1-siRNA and SIRT2-siRNA increased acetyl-α-tubulin level, conversely, over expression of SIRTs resulted in decreased the ratio of acetyl-α-tubulin to α-tubulin. We also found knockdown of SIRT1 and SIRT2 induced overexpression of βIV-tubulin and tubulin polymerization promoting protein (TPPP) (OLG-specific cytoskeleton-related molecules) that distributed widely in cell bodies. Taken together, SIRT1 may play a role in oligodenroglial differentiation and myelinogenesis. |
| Databáze: | OpenAIRE |
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