Sister chromatid exchanges and ion release in patients wearing fracture fixation devices
| Autor: | Donatella Granchi, Giuseppe Rollo, Aldo Toni, Lucia Savarino, Arturo Pizzoferrato, M. Elena Donati, Lucio Montanaro, Susanna Stea, Gabriela Ciapetti, M. Visentin, Gianfranco Zinghi |
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| Rok vydání: | 2000 |
| Předmět: |
Adult
Chromium Male medicine.medical_specialty Adolescent Biomedical Engineering chemistry.chemical_element Sister chromatid exchange Bone Nails medicine.disease_cause Biomaterials Nickel Reference Values Fracture fixation medicine Humans Lymphocytes Fixation (histology) Chemistry Spectrophotometry Atomic Radiochemistry Middle Aged Stainless Steel Orthopedic Fixation Devices Surgery Toxicity Female Implant Bone Plates Sister Chromatid Exchange Quantitative analysis (chemistry) Genotoxicity |
| Zdroj: | Journal of Biomedical Materials Research. 50:21-26 |
| ISSN: | 1097-4636 0021-9304 |
| DOI: | 10.1002/(sici)1097-4636(200004)50:1<21::aid-jbm4>3.0.co;2-r |
| Popis: | The quantification of sister chromatid exchange (SCE) during mitosis is a useful index for evaluating genotoxic effects in subjects occupationally or incidentally exposed to potentially toxic substances. The authors investigated the hypothesis that ions released by corrosion from prosthetic components of fracture fixation devices are associated with change in SCE incidence. In the present study, ten patients with implants were examined, and fifteen subjects with no implants were used as controls. SCE and high frequency cell (HFC) numbers were evaluated in circulating lymphocytes. In addition, nickel (Ni) and chromium (Cr) ion values in the serum were measured because, after iron, these metals are major components of stainless steel. A significant increase in SCE numbers was observed in patients compared to the control population (4.9 ± 1.3 vs. 3.5 ± 1.4). Ni concentration was 1.71 ± 1.49 ng/mL in patients and 0.72 ± 0.52 ng/mL in control subjects; Cr concentration was, respectively, 1.01 ± 0.77 ng/mL and 0.19 ± 0.27 ng/mL. The increase of serum Cr and Ni was statistically significant. No correlation was found between the increased Cr concentrations and SCE number while Cr ion levels were found to be significantly correlated to HFC. An inverse correlation between Ni level and SCE numbers was observed. Our findings suggest that Cr release by stainless steel implants could have a genotoxic effect; thus it would be useful to carefully monitor implanted subjects with regard to serum ion dosage, SCE analysis, and HFC evaluation. In any case, it would be appropriate to remove the implant when fracture fixation is reached. © 2000 John Wiley & Sons, Inc. J Biomed Mater Res, 50, 21–26, 2000. |
| Databáze: | OpenAIRE |
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