Basic fibroblast growth factor eluting microspheres enhance distraction enterogenesis
| Autor: | James C.Y. Dunn, Andrew Scott, Elvin Chiang, Shant Shekherdimian, Joshua D. Rouch, Steven L. Lee, Benjamin M. Wu, Ziyad Jabaji |
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| Rok vydání: | 2016 |
| Předmět: |
Short Bowel Syndrome
medicine.medical_specialty Angiogenesis Polyesters Basic fibroblast growth factor Tissue Expansion Jejunum Rats Sprague-Dawley 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Vascularity medicine Animals Tissue Scaffolds business.industry Regeneration (biology) Microvascular Density Tissue Expansion Devices General Medicine Short bowel syndrome medicine.disease Microspheres Surgery Fibroblast Growth Factors PLGA medicine.anatomical_structure chemistry 030220 oncology & carcinogenesis Pediatrics Perinatology and Child Health Models Animal 030211 gastroenterology & hepatology Female medicine.symptom business Biomedical engineering |
| Zdroj: | Journal of pediatric surgery. 51(6) |
| ISSN: | 1531-5037 |
| Popis: | Purpose The purpose of this study was to determine if distraction enterogenesis using self-expanding polycaprolactone (PCL) springs is a potential therapy for short bowel syndrome. Sustained release basic fibroblast growth factor (bFGF) microspheres have been shown to induce angiogenesis and intestinal regeneration in tissue engineered scaffolds. We hypothesized that the provision of bFGF-loaded microspheres would increase angiogenesis and thereby enhance the process of enterogenesis. Methods A 10-mm segment of rodent jejunum was isolated and an encapsulated PCL spring inserted. Blank or bFGF-loaded microspheres were delivered to the segment. After 4 weeks, jejunal segments were assessed for lengthening, morphology, quantification of blood vessels, and ganglia. Results Lengthened intestinal segments receiving bFGF microspheres demonstrated significantly increased microvascular density compared to those with blank microspheres. There were also significantly more submucosal and myenteric ganglia in the segments that received bFGF microspheres. Segments achieved similar lengthening and final muscular thickness in both blank and bFGF groups, but the bFGF microsphere caused a significant increase in luminal diameter of the jejunal segment. Conclusion Sustained release bFGF microspheres enhanced distraction enterogenesis through improved vascularity. The synergy of growth factors such as bFGF with distraction enterogenesis may yield improved results for the future treatment of patients with short bowel syndrome. |
| Databáze: | OpenAIRE |
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