Coordinated Expression of HPV-6 Genes with Predominant E4 and E5 Expression in Laryngeal Papilloma
| Autor: | Asanori Kiyuna, Hitoshi Hirakawa, Masatomo Touyama, Akira Ganaha, Hiroyuki Maeda, Shunsuke Kondo, Takayoshi Kosugi, Norimoto Kise, Narumi Hasegawa, Shinya Agena, Hidetoshi Kinjyo, Taro Ikegami, Akikazu Murakami, Narutoshi Tsukahara, Mikio Suzuki |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Microbiology (medical) human papillomavirus 6 long-term alteration of viral load Biology medicine.disease_cause Microbiology Article laryngeal papilloma 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Virology medicine lcsh:QH301-705.5 anti-E4 monoclonal antibody Gene viral mRNA expression RNA medicine.disease Molecular biology Immunosurveillance 030104 developmental biology lcsh:Biology (General) chemistry Viral replication 030220 oncology & carcinogenesis viral lineage Papilloma Carcinogenesis Viral load DNA |
| Zdroj: | Microorganisms Volume 9 Issue 3 Microorganisms, Vol 9, Iss 520, p 520 (2021) |
| ISSN: | 2076-2607 |
| Popis: | Laryngeal papilloma (LP) associated with human papillomavirus (HPV)-6 or -11 infection shows aggressive growth. However, the detailed molecular mechanism of virus-driven tumorigenesis has not been uncovered fully. HPV-6 viral gene expression and dynamic alterations were investigated with in situ localization of viral DNA and RNA in 13 patients with HPV-6-infected laryngeal papilloma. The average viral load was 4.80 × 105 ± 1.86 × 105 copies/ng DNA. E4, E5a, and E5b mRNAs accounted for 96% of the expression of 9 mRNAs. The alteration of viral DNA load during recurrence paralleled the mRNA expression levels, and the expression of all mRNAs showed a similar curve. E4, E5a, and E5b were expressed in the middle to upper part of the epithelium and were co-expressed in the same cells. E4 immunohistochemistry demonstrated an extensively positive reaction in the upper cell layer in accordance with E4 mRNA expression. These results suggest that individual viral genes are coordinately expressed for viral replication, virus release, and immunosurveillance avoidance. The newly developed E4-specific monoclonal antibody can be applied to further functional studies and clinical applications such as targeted molecular therapies. |
| Databáze: | OpenAIRE |
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