Bioactivity in SBF versus trace element effects: The isolated role of Mg2+ and Zn2+ in osteoblast behavior
| Autor: | Euler Araujo dos Santos, Chantal Damia, Débora dos Santos Tavares, Lucas de Araújo Bastos Santana, Paulo Henrique Oliveira Junior |
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| Přispěvatelé: | Federal University of Sergipe, Institut de Recherche sur les CERamiques (IRCER), Institut des Procédés Appliqués aux Matériaux (IPAM), Université de Limoges (UNILIM)-Université de Limoges (UNILIM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), IRCER - Axe 4 : céramiques sous contraintes environnementales (IRCER-AXE4), Université de Limoges (UNILIM)-Université de Limoges (UNILIM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut des Procédés Appliqués aux Matériaux (IPAM) |
| Rok vydání: | 2021 |
| Předmět: |
Materials science
Biocompatibility Simulated body fluid Bioengineering 02 engineering and technology 010402 general chemistry 01 natural sciences Apatite [SPI.MAT]Engineering Sciences [physics]/Materials law.invention Biomaterials law medicine Solubility Crystallization Cell adhesion ComputingMilieux_MISCELLANEOUS Osteoblast [CHIM.MATE]Chemical Sciences/Material chemistry Adhesion 021001 nanoscience & nanotechnology 0104 chemical sciences medicine.anatomical_structure Mechanics of Materials visual_art Biophysics visual_art.visual_art_medium 0210 nano-technology |
| Zdroj: | Materials Science and Engineering: C Materials Science and Engineering: C, Elsevier, 2021, 118, pp.111320. ⟨10.1016/j.msec.2020.111320⟩ |
| ISSN: | 0928-4931 |
| Popis: | The bioactivity assay originally proposed by Kokubo is one of the most commonly used tests to indirectly evaluate the biocompatibility of bioactive glasses. However, extensive evidence has shown that trace elements present in biomaterials may stimulate cellular behavior in different ways even when no apatite formation is observed, i.e., in biomaterials with low or no bioactivity. To further elucidate this topic, we designed three different SiO2-rich bioglass compositions in which CaO was partially replaced by ZnO and MgO, two oxides known to affect bioactivity as well as osteoblastic behavior. The physicochemical changes induced by the presence of oxides and their effects on biological behavior, as well as the adhesion, proliferation and differentiation of human osteoblast-like osteosarcoma cells (MG-63), were followed by a bioactivity assay in simulated body fluid (SBF). The insertion of ZnO or MgO decreased the glass transition (Tg) and crystallization (Tc) temperatures as a function of the increase in nonbonding oxygens, which was directly reflected in the higher solubility. The release of Mg2+ ions from the MgO-containing samples inhibited the bioactivity in SBF, inducing high cell adhesion and proliferation and moderate ALP activity. The release of Zn2+ also inhibited the bioactivity in SBF but, in contrast to the release of Mg2+, induced low cell adhesion and proliferation and high ALP activity compared to the control. |
| Databáze: | OpenAIRE |
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