An optofluidic constriction chip for monitoring metastatic potential and drug response of cancer cells
| Autor: | Tie Yang, Francesca Bragheri, Roberto Osellame, R. Martinez Vazquez, Paolo Minzioni, Chiara Mondello, M. Di Tano, Ilaria Cristiani, Giovanni Nava, Ilaria Chiodi, M. Veglione, Elena Bianchi |
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| Rok vydání: | 2015 |
| Předmět: |
Cell
Drug Evaluation Preclinical Biophysics paclitaxe Antineoplastic Agents Apoptosis Cell Separation Biochemistry Flow cytometry chemistry.chemical_compound Cell Movement Lab-On-A-Chip Devices medicine metastasis combretastatin A-4 optofluidic chip Combretastatin medicine.diagnostic_test Chemistry optofluidics Optical Devices cytoskeleton Equipment Design Neoplasms Experimental Flow Cytometry medicine.disease Metastatic breast cancer Equipment Failure Analysis Nocodazole nocodazole medicine.anatomical_structure Paclitaxel Flow Injection Analysis Cancer cell cancer cells Cancer research Biological Assay |
| Zdroj: | Integrative biology (Camb., Online) 7 (2015): 477–484. doi:10.1039/C5IB00023H info:cnr-pdr/source/autori:Martinez Vazquez R, Nava G, Veglione M, Yang T, Bragheri F, Minzioni P, Bianchi E, Di Tano M, Chiodi I, Osellame R, Mondello C, Cristiani I./titolo:An optofluidic constriction chip for monitoring metastatic potential and drug response of cancer cells/doi:10.1039%2FC5IB00023H/rivista:Integrative biology (Camb., Online)/anno:2015/pagina_da:477/pagina_a:484/intervallo_pagine:477–484/volume:7 Integrative biology (Camb., Print) 7 (2015): 477–484. doi:10.1039/c5ib00023h info:cnr-pdr/source/autori:Vazquez, R. Martinez; Nava, G.; Veglione, M.; Yang, T.; Bragheri, F.; Minzioni, P.; Bianchi, E.; Di Tano, M.; Chiodi, I.; Osellame, R.; Mondello, C.; Cristiani, I./titolo:An optofluidic constriction chip for monitoring metastatic potential and drug response of cancer cells/doi:10.1039%2Fc5ib00023h/rivista:Integrative biology (Camb., Print)/anno:2015/pagina_da:477/pagina_a:484/intervallo_pagine:477–484/volume:7 |
| ISSN: | 1757-9708 1757-9694 |
| DOI: | 10.1039/c5ib00023h |
| Popis: | Cellular mechanical properties constitute good markers to characterize tumor cells, to study cell population heterogeneity and to highlight the effect of drug treatments. In this work, we describe the fabrication and validation of an integrated optofluidic chip capable of analyzing cellular deformability on the basis of the pressure gradient needed to push a cell through a narrow constriction. We demonstrate the ability of the chip to discriminate between tumorigenic and metastatic breast cancer cells (MCF7 and MDA-MB231) and between human melanoma cells with different metastatic potential (A375P and A375MC2). Moreover, we show that this chip allows highlighting the effect of drugs interfering with microtubule organization (paclitaxel, combretastatin A-4 and nocodazole) on cancer cells, which leads to changes in the pressure-gradient required to push cells through the constriction. Our single-cell microfluidic device for mechanical evaluation is compact and easy to use, allowing for an extensive use in different laboratory environments. |
| Databáze: | OpenAIRE |
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