Novel Technique for Isolating Human Bone Marrow Stem Cells Using Hyaluronic Acid Hydrogel
| Autor: | Jae Kook Cha, Byung Joo Jung, Seong Bok Choi, Chang-Sung Kim, Jung Seok Lee, Eun Young Choi, Seul Ki Kim |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Adult
Male 0301 basic medicine Density gradient Biomedical Engineering Medicine (miscellaneous) Bone Marrow Cells Bioengineering Cell Separation Hydrogel Polyethylene Glycol Dimethacrylate Extracellular matrix 03 medical and health sciences chemistry.chemical_compound Hyaluronic acid Humans Regeneration Centrifugation Hyaluronic Acid Cells Cultured Tissue Engineering Regeneration (biology) Mesenchymal stem cell Cell Differentiation Mesenchymal Stem Cells Middle Aged Extracellular Matrix Transplantation 030104 developmental biology chemistry Female Stem cell Biomedical engineering |
| Zdroj: | Tissue Engineering Part C: Methods. 22:941-951 |
| ISSN: | 1937-3392 1937-3384 |
| DOI: | 10.1089/ten.tec.2016.0214 |
| Popis: | Centrifugation based on density gradients is a general methodology for isolating human bone marrow (hBM)-derived mesenchymal stem cells (hBMSCs). The mononuclear cell (MNC) layer can be obtained using a density gradient solution in the conventional protocol, but it is not suitable for direct transplantation due to the possible toxicity of this solution. The results obtained are also influenced by the skill level when applying the technique, which involves time-consuming processes. We have developed a novel protocol for isolating hBMSCs using hyaluronic acid (HA), which is the most widely used injectable biomaterial in clinical settings and a major component of the extracellular matrix. Laying hBM over the HA and then applying centrifugation yielded three separate layers, with the HA layer, including MNCs being the most superficial one. Increasing the volume of HA and/or its crosslinking rate enhanced the yield of MNCs from hBM, and the cell yield was also significantly higher for a lower centrifugal acceleration (530 g) than for a higher one (1500 g). Isolated hBMSCs by HA exhibited similar biological characteristics such as in terms of their proliferation rate, fibroblast-like morphology, cell-cycle status, immunophenotype, and multipotency. The use of either type of hBMSC confirmed the regenerative potential of bone and bone marrow-like tissue in ectopic transplantation models. This is the first report of a novel protocol for isolating hBMSCs that utilize HA. We suggest that this novel isolation technique can be used for the direct application of autogenous MSCs with advantages of being less time-consuming and involving steps that are easier to perform. |
| Databáze: | OpenAIRE |
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