High sensitivity of C. elegans vulval precursor cells to the dose of posterior Wnts
| Autor: | Jean-Baptiste Pénigault, Marie-Anne Félix |
|---|---|
| Přispěvatelé: | Institut Jacques Monod (IJM (UMR_7592)), Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique, Association pour la Recherche sur le Cancer (#1044) |
| Rok vydání: | 2011 |
| Předmět: |
MESH: Signal Transduction
Cell Haploinsufficiency 0302 clinical medicine MESH: Animals Hox gene [SDV.BDD]Life Sciences [q-bio]/Development Biology Caenorhabditis elegans Cell competence level Genetics 0303 health sciences biology Stem Cells Wnt signaling pathway MESH: Caenorhabditis elegans Proteins Hox Cell biology MESH: Wnt Proteins medicine.anatomical_structure Larva MESH: Cell Division Body region Female Cell Division MESH: Body Patterning Wnt gradient Protein Binding Signal Transduction Gonad C. elegans vulva MESH: Stem Cells Models Biological Vulva 03 medical and health sciences MESH: Gonads Precursor cell MESH: Caenorhabditis elegans medicine MESH: Protein Binding Animals Caenorhabditis elegans Proteins Gonads Gene Molecular Biology 030304 developmental biology Body Patterning fungi MESH: Models Biological Cell Biology biology.organism_classification Wnt Proteins MESH: Vulva MESH: Female MESH: Larva 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Developmental Biology Developmental Biology, Elsevier, 2011, 357 (2), pp.428-38. ⟨10.1016/j.ydbio.2011.06.006⟩ |
| ISSN: | 1095-564X 0012-1606 |
| DOI: | 10.1016/j.ydbio.2011.06.006⟩ |
| Popis: | International audience; Cell competence is a key developmental property. The Caenorhabditis elegans vulval competence group consists of P(3-8).p, six cells aligned along the antero-posterior axis in a wide central body region. The six cells are not equal in their competence: 1) P3.p quits the competence group in half of the individuals; 2) the posterior cells P7.p and P8.p are less competent than central vulval precursor cells. Competence to adopt a vulval fate is controlled by expression of the HOM-C gene lin-39, and maintained through Wnt signals that are secreted from the tail in a long-range gradient. Here we quantify the LIN-39 protein profile in vulval precursor cells of early L2 stage larvae, prior to P3.p fusion and inductive signaling. We show that LIN-39 levels are very low in P3.p and P4.p, peak in P5.p and progressively decrease until P8.p. This unexpectedly centered profile arises independently from the gonad. Posterior Wnt signaling reduces LIN-39 level in the posterior cells by activating the next-posterior HOM-C gene, mab-5. On the anterior side, P3.p and P4.p competence and division are sensitive to the already low LIN-39 and Wnt doses; most dramatically, each of the cwn-1/Wnt and egl-20/Wnt genes show haplo-insufficience for P3.p fate. In contrast to previous results, we find that these Wnts maintain P3.p and P4.p competence without affecting their LIN-39 level. The centered vulval competence profile is thus under the control of the posterior Wnts and of cross-regulation of three HOM-C genes and prepatterns the later induction of vulval fates. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect