Attenuation of the cyproterone acetate-induced testicular hypofunction by a novel nutraceutical lycopene: a genomic approach
| Autor: | Adrija Tripathy, Debidas Ghosh, Bhabani Prasad Pakhira, Abhinandan Ghosh, A. Dey |
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| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine endocrine system medicine.medical_specialty Cell Survival Urology Biology Male infertility 03 medical and health sciences chemistry.chemical_compound Lycopene 0302 clinical medicine Endocrinology Oral administration Malondialdehyde Internal medicine Testis medicine Animals Cyproterone Acetate Spermatogenesis Sperm motility 030219 obstetrics & reproductive medicine Sperm Count Superoxide Dismutase urogenital system Cyproterone acetate Genomics General Medicine Catalase medicine.disease Carotenoids Spermatozoa Sperm Rats Oxidative Stress 030104 developmental biology chemistry Dietary Supplements Sperm Motility |
| Zdroj: | Andrologia. 49:e12709 |
| ISSN: | 0303-4569 |
| Popis: | Summary This study was designed to explore the cyproterone acetate (CPA)-induced andrological hypofunction and its correction by oral administration of lycopene. In this concern, spermatogenic, biochemical, histological and genomic profiles were studied. Cyproterone acetate administration for 1 month helped to develop infertile model rats. A significant recovery was noted in sperm motility, sperm count, sperm viability, hypo-osmotic swelling tail-coiled spermatozoa; activities of testicular ∆5, 3β-hydroxysteroid dehydrogenase (HSD), 17β-HSD, catalase (CAT) and superoxide dismutase (SOD); and levels of conjugated diene (CD), malondialdehyde (MDA), testicular cholesterol and serum testosterone after the administration of lycopene at 1.5 mg/0.5 ml Tween-80/100 g body weight/day for last 1 month to infertile model rats. Simultaneously, qRT-PCR study of Bax, Bcl-2, caspase-3, ∆5, 3β-HSD and 17β-HSD genes in testicular tissue showed a significant rectification towards the control in CPA-pre-treated cum CPA–lycopene-cotreated rats. Side-by-side histological and histometric studies showed a significant correction in qualitative analysis of spermatogenesis and seminiferous tubular diameter (STD) in CPA-pre-treated cum CPA–lycopene-cotreated rats. Lycopene showed outstanding efficacy in the management of CPA-induced testicular hypofunction with special reference to correction in oxidative stress-induced testicular apoptosis at genomic level. |
| Databáze: | OpenAIRE |
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