Combination Effect of Photodynamic and Sonodynamic Therapy on Experimental Skin Squamous Cell Carcinoma in C3H/HeN Mice
| Autor: | Kazumori Ishiguro, Keigo Takaoka, Susumu Nakajima, Masaru Fukuda, Keiichi Ueda, Masanobu Kumakiri, Nagahiko Yumita, Norio Miyoshi, Isao Sakata, Zhao Hui Jin, Shin-ichiro Umemura, Takeshi Udagawa, Hisao Tajiri, Kenichi Kawabata |
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| Rok vydání: | 2000 |
| Předmět: |
Chlorophyll
Male medicine.medical_specialty Porphyrins Skin Neoplasms Combination therapy Ultrasonic Therapy medicine.medical_treatment Gallium Photodynamic therapy Dermatology Mice Random Allocation Skin Squamous Cell Carcinoma Carcinoma Animals Medicine Combined Modality Therapy Photosensitizer Survival analysis Mice Inbred C3H Photosensitizing Agents business.industry Sonodynamic therapy General Medicine medicine.disease Survival Analysis Surgery Disease Models Animal Photochemotherapy Carcinoma Squamous Cell Cancer research Female business |
| Zdroj: | The Journal of Dermatology. 27:294-306 |
| ISSN: | 0385-2407 |
| DOI: | 10.1111/j.1346-8138.2000.tb02171.x |
| Popis: | We studied a combination of photodynamic therapy (PDT) and sonodynamic therapy (SDT) for improving tumoricidal effects in a transplantable mouse squamous cell carcinoma (SCC) model. Two sensitizers were utilized: the pheophorbide-a derivative PH-1126, which is a newly developed photosensitizer, and the gallium porphyrin analogue ATX-70, a commonly used sonosensitizer. Mice were injected with either PH-1126 or ATX-70 i.p. at doses of 5 or 10 mg/kg.bw. At 24 (ATX-70) or 36 hr (PH-1126) (time of optimum drug concentration in the tumor) after injection, SCCs underwent laser light irradiation (88 J/cm2 of 575 nm for ATX-70; 44J/cm2 of 650 nm for PH-1126) (PDT), ultrasound irradiation (0.51 W/cm2 at 1.0 MHz for 10 minutes) (SDT), or a combination of the two treatments. The combination of PDT and SDT using either PH-1126 or ATX-70 as a sensitizer resulted in significantly improved inhibition of tumor growth (92-98%) (additive effect) as compared to either single treatment (27-77%). The combination using PH-1126 resulted in 25% of the treated mice being tumor free at 20 days after treatment. Moreover, the median survival period (from irradiation to death) of PDT + SDT-treated mice (> 120 days) was significantly greater than that in single treatment groups (77-95 days). Histological changes revealed that combination therapy could induce tumor necrosis 2-3 times as deep as in either of the single modalities. The combination of PDT and SDT could be very useful for treatment of non-superficial or nodular tumors. |
| Databáze: | OpenAIRE |
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