Morphologic and immunohistochemical characterization of granulomas in the nucleotide oligomerization domain 2-related disorders Blau syndrome and Crohn disease
| Autor: | Carlos D. Rose, Rebecca ten Cate, Gert De Hertogh, Valeer Desmet, Tammy M. Martin, Brigitte Bader Meunier, Rolando Cimaz, Caroline Thomee, Tania Roskams, Pierre Quartier, Alain Fischer, Miroslav Harjacek, Carl E.I. Janssen, Carine Wouters |
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| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Male
Pathology medicine.medical_specialty multinucleated giant cell Adolescent Sarcoidosis Receptor expression Immunology Nod2 Signaling Adaptor Protein Biology Nucleotide oligomerization domain 2 Uveitis hemic and lymphatic diseases NOD2 Biopsy medicine Humans Immunology and Allergy Fibrinoid necrosis Child granuloma Blau syndrome monocyte macrophage lineage Synovitis medicine.diagnostic_test Arthritis Infant Crohn disease medicine.disease Immunohistochemistry Cranial Nerve Diseases Giant cell Child Preschool Granuloma Mutation Cytokines Female T(H)17 cell Epithelioid cell |
| Popis: | Background Blau syndrome (BS) and Crohn disease (CD) are both characterized by granulomatous inflammation and related to nucleotide oligomerization domain 2 (NOD2) mutations. Objective This study aimed to define the morphologic and immunohistochemical characteristics of granulomas in patients with NOD2 -related BS and CD. Methods Granuloma-containing biopsy specimens from 6 patients with BS and 7 pediatric patients with CD carrying NOD2 mutations or single nucleotide polymorphisms were studied for morphology, cellular composition, and cytokine expression by using hematoxylin and eosin staining and immunohistochemistry. Results Biopsy specimens from patients with BS typically showed polycyclic granulomas with large lymphocytic coronas, extensive emperipolesis of lymphocytes within multinucleated giant cells (MGCs), MGC death, and fibrinoid necrosis and fibrosis. In contrast, biopsy specimens from patients with CD showed simple granulomas with subtle/absent lymphocytic coronas, sclerosis of the surrounding tissue, and polymorphonuclear cells. Findings found to be similar in all granulomas were as follows: CD68 and HLA-DR expression by epithelioid cells, monocyte-macrophage lineage cells and MGCs, increased lymphocytic HLA-DR expression, increased CD4 + /CD8 + T-cell ratio, and CD20 + B lymphocytes evenly distributed within and around granulomas. In both patient groups prominent IFN-γ expression was found in and around granulomas, and TNF-α and IL-23 receptor expression was moderate. IL-6, IL-17, and TGF-β expression was prominent in granulomas from patients with BS but sporadic in granulomas from patients with CD. IL-10 expression was absent. Conclusion Granulomas from patients with BS and granulomas from patients with NOD2 -associated CD show distinct morphologic features and cytokine expression patterns, suggesting that the T H 17 axis might be involved in the pathogenesis of BS, whereas T H 1 is important in both patients with BS and patients with CD. |
| Databáze: | OpenAIRE |
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