Structural insights into Arabidopsis ethylene response factor 96 with an extended N-terminal binding to GCC box
| Autor: | Kun-Hung Chen, Pei-Hsuan Lin, Yi-Sheng Cheng, Chun-Yen Chen |
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| Rok vydání: | 2020 |
| Předmět: |
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Molecular EDLL motif DNA Plant Protein Conformation AP2/ERF Regulator Arabidopsis Plant Science Biology Crystallography X-Ray Article Transactivation chemistry.chemical_compound Protein structure ERF 96 Genetics Gene Transcription factor Binding Sites Arabidopsis Proteins Protoplasts fungi food and beverages GCC box General Medicine biology.organism_classification Cell biology Fluorescence polarization assay chemistry Mutation Sequence motif Agronomy and Crop Science DNA Transcription Factors |
| Zdroj: | Plant Molecular Biology |
| ISSN: | 1573-5028 |
| Popis: | The phytohormone ethylene is widely involved in many developmental processes and is a crucial regulator of defense responses against biotic and abiotic stresses in plants. Ethylene-responsive element binding protein, a member of the APETALA2/ethylene response factor (AP2/ERF) superfamily, is a transcription factor that regulates stress-responsive genes by recognizing a specific cis-acting element of target DNA. A previous study showed only the NMR structure of the AP2/ERF domain of AtERF100 in complex with a GCC box DNA motif. In this report, we determined the crystal structure of AtERF96 in complex with a GCC box at atomic resolution. We analyzed the binding residues of the conserved AP2/ERF domain in the DNA recognition sequence. In addition to the AP2/ERF domain, an N-terminal α-helix of AtERF96 participates in DNA interaction in the flanking region. We also demonstrated the structure of AtERF96 EDLL motif, a unique conserved motif in the group IX of AP2/ERF family, might involve in the transactivation of defense-related genes. Our study establishes the structural basis of the AtERF96 transcription factor in complex with the GCC box, as well as the DNA binding mechanisms of the N-terminal α-helix and AP2/ERF domain. Electronic supplementary material The online version of this article (doi:10.1007/s11103-020-01052-5) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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