Effects of different culture systems on the culture of prepuberal buffalo (Bubalus bubalis) spermatogonial stem cell-like cells in vitro
| Autor: | Huan Yang, Xiao Gan Yang, Shuang Shuang Geng, Ke Huan Lu, Xing Wei Liang, Huiyan Xu, Yang Qing Lu, Li Tingting, Peng Wei Zhao, Ao Lin Luo |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Male
Buffaloes 040301 veterinary sciences Cell Culture Techniques Retinoic acid Buffalo Biology 0403 veterinary science Andrology 03 medical and health sciences chemistry.chemical_compound Gene expression Animals Spermatogonial stem cells Bovine serum albumin Cells Cultured 030304 developmental biology spermatogonial stem cells 0303 health sciences Adult Germline Stem Cells General Veterinary Cell Biology 04 agricultural and veterinary sciences biology.organism_classification Spermatogonia In vitro chemistry Cell culture biology.protein Original Article Bubalus Stem cell primary cell culture |
| Zdroj: | Journal of Veterinary Science |
| ISSN: | 1976-555X 1229-845X |
| DOI: | 10.4142/jvs.2020.21.e13 |
| Popis: | Currently, the systems for culturing buffalo spermatogonial stem cells (SSCs) in vitro are varied, and their effects are still inconclusive. In this study, we compared the effects of culture systems with undefined (foetal bovine serum) and defined (KnockOut Serum Replacement) materials on the in vitro culture of buffalo SSC-like cells. Significantly more DDX4- and UCHL1-positive cells (cultured for 2 days at passage 2) were observed in the defined materials culture system than in the undefined materials system (p < 0.01), and these cells were maintained for a longer period than those in the culture system with undefined materials (10 days vs. 6 days). Furthermore, NANOS2 (p < 0.05), DDX4 (p < 0.01) and UCHL1 (p < 0.05) were expressed at significantly higher levels in the culture system with defined materials than in that with undefined materials. Induction with retinoic acid was used to verify that the cultured cells maintained SSC characteristics, revealing an SCP3+ subset in the cells cultured in the defined materials system. The expression levels of Stra8 (p < 0.05) and Rec8 (p < 0.01) were significantly increased, and the expression levels of ZBTB16 (p < 0.01) and DDX4 (p < 0.05) were significantly decreased. These findings provided a clearer research platform for exploring the mechanism of buffalo SSCs in vitro. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|