IncP-1ε plasmids are important vectors of antibiotic resistance genes in agricultural systems: diversification driven by class 1 integron gene cassettes
| Autor: | Thomas Ledger, Eva M. Top, Bernardo González, Sven Jechalke, Kornelia Smalla, Chu Thi Thanh Binh, Christoph Kopmann, Holger Heuer, Ellen Krögerrecklenfort, Ute Zimmerling |
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| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Microbiology (medical)
Transposable element antibiotic resistance Tetracycline lcsh:QR1-502 gene cassette Integron Microbiology lcsh:Microbiology 03 medical and health sciences Plasmid Gammaproteobacteria complete sequence medicine Original Research 030304 developmental biology 2. Zero hunger Genetics 0303 health sciences exogenous isolation biology 030306 microbiology biology.organism_classification Manure arable soil pig manure qPCR Gene cassette Horizontal gene transfer IncP-1ε plasmid biology.protein medicine.drug |
| Zdroj: | Frontiers in Microbiology, Vol 3 (2012) Frontiers in Microbiology |
| DOI: | 10.3389/fmicb.2012.00002/full |
| Popis: | The role of broad host range IncP-1ε plasmids in the dissemination of antibiotic resistance in agricultural systems has not yet been investigated. These plasmids were detected in total DNA from all of 16 manure samples and in arable soil based on a novel 5’-nuclease assay for real time PCR. A correlation between IncP-1ε plasmid abundance and antibiotic usage was revealed. In a soil microcosm experiment the abundance of IncP-1ε plasmids was significantly increased even 127 days after application of manure containing the antibiotic compound sulfadiazine, compared to soil receiving only manure, only sulfadiazine, or water. Fifty IncP-1ε plasmids that were captured in E. coli CV601gfp from bacterial communities of manure and arable soil were characterized by PCR and hybridisation. All plasmids carried class 1 integrons with highly varying sizes of the gene cassette region and the sul1 gene. Three IncP-1ε plasmids captured from soil bacteria and one from manure were completely sequenced. The backbones were nearly identical to that of the previously described IncP-1ε plasmid pKJK5. The plasmids differed mainly in the composition of a Tn402-like transposon carrying a class 1 integron with varying gene cassettes, IS1326, and in three of the plasmids the tetracycline resistance transposon Tn1721 with various truncations. Diverse Beta- and Gammaproteobacteria were revealed as hosts of one of the IncP-1ε plasmids in soil microcosms. Our data suggest that IncP-1ε plasmids are important vectors for horizontal transfer of antibiotic resistance in agricultural systems. |
| Databáze: | OpenAIRE |
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