Gold and Gold-Palladium Coated Polypropylene Grafts in a S. epidermidis Wound Infection Model
| Autor: | Sabahat Çeken, Cagatay Daphan, Canan Agalar, Meral Saygun, Abdullah Akkus, Oral Saygun, Fatih Agalar, Emir Baki Denkbaş, Kuzey Aydinuraz |
|---|---|
| Přispěvatelé: | Kırıkkale Üniversitesi |
| Rok vydání: | 2006 |
| Předmět: |
Pathology
medicine.medical_specialty medicine.medical_treatment Hernia Inguinal Bacterial growth Polypropylenes Agar plate Abdominal wall chemistry.chemical_compound Staphylococcus epidermidis medicine Animals Surgical Wound Infection Rats Wistar foreign body infection Incubation Saline Polypropylene biology Abdominal Wall Staphylococcal Infections Surgical Mesh gold Foreign Bodies palladium biology.organism_classification Rats S. epidermidis Surgery Surgical mesh medicine.anatomical_structure chemistry Biofilms Female polypropylene |
| Zdroj: | Journal of Surgical Research. 131:73-79 |
| ISSN: | 0022-4804 |
| Popis: | WOS: 000235771200010 PubMed: 16139304 Background. The use of non-absorbable mesh grafts in both abdominal wall defects and inguinal hernias are impossible in the presence of contamination. This study was conducted for evaluation of the efficiencies of polypropylene mesh grafts coated with gold and palladium-gold. Materials and methods. Ten piece of 1 x 2 cm. of polypropylene mesh grafts were used in each group of naive, gold-coated, and palladium-gold-coated. The grafts were incubated in physiological saline buffered and 0.5 McFarland slime positive Staphylococcus epidermidis for 24 h. At intervals of 6,12,24,48, 72 h grafts were washed with saline and vortexed for 2 min in 2 ml of physiological saline. There were 100 mu l of samples of vortexed material incubated in blood agar and 24 h later, colony numbers were assessed. In the second part of study, the grafts were implanted below the musculoaponeurotic layer at inguinal. region of rats following the same procedure of incubation and washing. On the 8th day, the rats were examined for infection rate and their wound cultures were obtained. Results. The least amount of bacterial growth was detected in the samples obtained from gold-palladium coated grafts; whereas the highest rate of growth was found in samples of naive grafts. The superficial surgical site infection rate was 0% in gold-palladium coated, 30% in gold-coated and 100% in naive polypropylene group. The bacterial growth rate from wound cultures confirmed the superficial surgical site infection rates in all groups. Conclusion. Prosthetic graft infection with S. epidermidis can be prevented by coating the graft with gold-palladium or gold. (c) 2006 Elsevier Inc. All rights reserved. |
| Databáze: | OpenAIRE |
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