The effects of oxidised phospholipids and cholesterol on the biophysical properties of POPC bilayers
| Autor: | Megan L. O'Mara, Alexandra Schumann-Gillett |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Lipid Bilayers
Biophysics Phospholipid Molecular Conformation Molecular Dynamics Simulation 01 natural sciences Biochemistry 03 medical and health sciences chemistry.chemical_compound Lipid oxidation 0103 physical sciences Lipid bilayer POPC Phospholipids 030304 developmental biology 0303 health sciences 010304 chemical physics Cholesterol Bilayer Cell Membrane Biological membrane Cell Biology Lipids Oxygen Membrane chemistry Phosphatidylcholines lipids (amino acids peptides and proteins) Signal Transduction |
| Zdroj: | Biochimica et biophysica acta. Biomembranes. 1861(1) |
| ISSN: | 1879-2642 |
| Popis: | Oxidation of unsaturated membrane phospholipids by oxidative stress is associated with inflammation, infection, numerous diseases and neurodegenerative disorders. Lipid oxidation is observed in experimental samples when the parent lipid is exposed to oxidative stressors. The effect of phospholipid oxidation on the properties of biological membranes are still being explored, while low concentrations (0.1–2.0 mol%) of oxidised phospholipids are associated with disease states [ 1 ]. Previous computational studies have focused on the effect of high concentrations (~50 mol%) of oxidised phospholipids on binary lipid bilayers. This work systematically characterises the effect of lower concentrations (~10 mol%) of two oxidised lipid species, PoxnoPC (1-palmitoyl-2-(9′-oxo-nonanoyl)- sn -glycero-3-phosphocholine) or PazePC (1-palmitoyl-2-azelaoyl- sn -glycero-3-phosphocholine), on POPC/cholesterol and pure POPC bilayers. During μs atomistic simulations in pure POPC bilayers, PoxnoPC and PazePC reoriented their oxidised sn -2 acyl chains towards the solution, and PazePC adopted an extended conformation. The addition of 20 mol% cholesterol not only modulated the fluidity of the bilayers; it also modulated the flexibility of the PoxnoPC oxidised sn -2 tail, reducing bilayer disorder. In contrast, the addition of cholesterol had little effect on bilayers containing PazePC. Our studies show that the effect of oxidised lipids on the biophysical properties of a multicomponent bilayer cannot be intuitively extrapolated from a binary lipid system. |
| Databáze: | OpenAIRE |
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