α-TERTHIENYL PHOTOSENSITIZES DAMAGE TO pBR322 DNA
| Autor: | R. W. Tuveson, Jacques Kagan, Tian P. Wang, G. R. Wang |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
DNA
Bacterial Antioxidant Photochemistry DNA damage medicine.medical_treatment Thiophenes Biochemistry Superoxide dismutase chemistry.chemical_compound Transformation Genetic medicine Physical and Theoretical Chemistry Histidine biology DNA Superhelical Chemistry General Medicine Molecular biology In vitro Transformation (genetics) Catalase biology.protein DNA DNA Damage Plasmids |
| Zdroj: | Photochemistry and Photobiology. 53:463-467 |
| ISSN: | 1751-1097 0031-8655 |
| DOI: | 10.1111/j.1751-1097.1991.tb03657.x |
| Popis: | alpha-Terthienyl photosensitizes single strand breaks in pBR322 DNA. Almost identical results were observed under oxygen and under argon. In the presence of oxygen, this DNA nicking was enhanced by histidine and was not affected by superoxide dismutase, catalase, or the antioxidant BHT. Although chemical damage to DNA treated with alpha-terthienyl plus near-UV was clearly demonstrated in vitro, transformation in E. coli with this damaged pBR322 DNA still took place. Likewise, Haemophilus influenzae DNA transforming activity was not significantly decreased by photosensitization with alpha-terthienyl. |
| Databáze: | OpenAIRE |
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