Molecular mechanisms in IL-1β-mediated decorin production by decidual cells
| Autor: | C D Halari, S J Renaud, P K Lala |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Embryology
Interleukin-1beta Active Transport Cell Nucleus Cell Line 03 medical and health sciences 0302 clinical medicine Pregnancy Decidua Genetics Humans Promoter Regions Genetic Molecular Biology 030304 developmental biology Receptors Interleukin-1 Type I 0303 health sciences Binding Sites 030219 obstetrics & reproductive medicine Macrophages NF-kappa B Obstetrics and Gynecology Cell Biology Up-Regulation carbohydrates (lipids) Pregnancy Trimester First Reproductive Medicine Female Decorin Stromal Cells Developmental Biology |
| Zdroj: | Molecular Human Reproduction. 27 |
| ISSN: | 1460-2407 1360-9947 |
| DOI: | 10.1093/molehr/gaab068 |
| Popis: | Decorin, a small leucine-rich proteoglycan produced by decidual cells restrains trophoblast differentiation, migration and invasiveness of extra-villous trophoblast cells. Decidual overproduction of decorin is associated with preeclampsia, and elevated decorin levels in maternal plasma are a predictive biomarker of preeclampsia. Furthermore, decorin plays an autocrine role in maturation of human endometrial stromal cells into decidual cells. Thus, a balanced decorin production by the decidua is critical for healthy pregnancy. However, the molecular mechanisms regulating decorin production by the decidua are unclear. Interleukin-1 beta is an inflammation-associated multi-functional cytokine, and is reported to induce decidualization in primates. Hence, the present study was designed: (i) to test if exogenous Interleukin-1 beta stimulated decorin production by human endometrial stromal cells; and if so, (ii) to identify the cellular source of Interleukin-1 beta in first trimester decidual tissue; (iii) to identify the downstream molecular partners in Interleukin-1 beta mediated decorin production by human endometrial stromal cells. Results revealed that (i) amongst multiple pro-inflammatory cytokines tested, Interleukin-1 beta alone stimulated decorin production by these cells; (ii) both macrophages and decidual cells in first trimester decidua produced Interleukin-1 beta; (iii) Interleukin-1 beta mediated decorin production was dependent on Interleukin-1 receptor activation, followed by activation and nuclear translocation of nuclear factor kappa B and its binding to the decorin promoter. These results reveal that Interleukin-1 beta plays a novel role in inducing decorin production by human endometrial stromal cells by activating nuclear factor kappa B. |
| Databáze: | OpenAIRE |
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