Mechano growth factor (MGF) and transforming growth factor (TGF)-β3 functionalized silk scaffolds enhance articular hyaline cartilage regeneration in rabbit model
| Autor: | Yuanliang Wang, Yan Xu, Haibin Li, Wei Xu, Shuangchi Wu, Ziwei Luo, Li Jiang, Li Yang, Yonggang Lv, Zhenyu Tang |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Cartilage
Articular Materials science Compressive Strength Silk Biophysics Fibroin Bioengineering Biomaterials Extracellular matrix Transforming Growth Factor beta3 Tissue engineering Spectroscopy Fourier Transform Infrared medicine Animals Humans Regeneration Cell Lineage Insulin-Like Growth Factor I Collagen Type II Aggrecan Hyaline cartilage Stem Cells Cartilage Fibrocartilage Cell Differentiation Mesenchymal Stem Cells Chondrogenesis Fibrosis Cell biology Hyaline Cartilage medicine.anatomical_structure Mechanics of Materials Microscopy Electron Scanning Ceramics and Composites Collagen Rabbits Stress Mechanical Fibroins Porosity Biomedical engineering |
| Zdroj: | Biomaterials. 52:463-475 |
| ISSN: | 0142-9612 |
| Popis: | Damaged cartilage has poor self-healing ability and usually progresses to scar or fibrocartilaginous tissue, and finally degenerates to osteoarthritis (OA). Here we demonstrated that one of alternative isoforms of IGF-1, mechano growth factor (MGF) acted synergistically with transforming growth factor β3 (TGF-β3) embedded in silk fibroin scaffolds to induce chemotactic homing and chondrogenic differentiation of mesenchymal stem cells (MSCs). Combination of MGF and TGF-β3 significantly increased cell recruitment up to 1.8 times and 2 times higher than TGF-β3 did in vitro and in vivo. Moreover, MGF increased Collagen II and aggrecan secretion of TGF-β3 induced hMSCs chondrogenesis, but decreased Collagen I in vitro. Silk fibroin (SF) scaffolds have been widely used for tissue engineering, and we showed that methanol treated pured SF scaffolds were porous, similar to compressive module of native cartilage, slow degradation rate and excellent drug released curves. At 7 days after subcutaneous implantation, TGF-β3 and MGF functionalized silk fibroin scaffolds (STM) recruited more CD29+/CD44+cells (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect